• Immunohistochemistry analysis of paraffin-embedded mouse brain using BRD9 antibody (STJ11105726) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using BRD9 antibody (STJ11105726) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using BRD9 antibody (STJ11105726) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-BRD9 antibody (274-597) (STJ11105726)

SKU:
STJ11105726

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Bromodomain-Containing Protein 9 (274-597) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BRD9
Gene ID: 65980
Uniprot ID: BRD9_HUMAN
Immunogen Region: 274-597
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 274-597 of human BRD9 (NP_076413.3).
Immunogen Sequence: REVISCMFEPEGNACSLTDS TAEEHVLALVEHAADEARDR INRFLPGGKMGYLKRNGDGS LLYSVVNTAEPDADEEETHP VDLSSLSSKLLPGFTTLGFK DERRNKVTFLSSATTALSMQ NNSVFGDLKSDEMELLYSAY GDETGVQCALSLQEFVKDAG SYSKKVVDDLLDQITGGDHS RTLFQLKQRRNVPMKPPDEA KVGDTLGDSSSSVLEFMSMK SYPDVSVDISMLSSLGKVK
Function Plays a role in chromatin remodeling and regulation of transcription. Acts as a chromatin reader that recognizes and binds acylated histones: binds histones that are acetylated and/or butyrylated. Component of SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Orchestrates also the RAD51-RAD54 complex formation and thereby plays a role in homologous recombination (HR).
Protein Name Bromodomain-Containing Protein 9
Rhabdomyosarcoma Antigen Mu-Rms-40.8
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Bromodomain-Containing Protein 9 antibody
Anti-Rhabdomyosarcoma Antigen Mu-Rms-40.8 antibody
Anti-BRD9 antibody
Anti-UNQ3040 antibody
Anti-PRO9856 antibody

Information sourced from Uniprot.org

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