• Immunohistochemistry analysis of BRAP in paraffin-embedded human liver cancer using BRAP Rabbit polyclonal antibody (STJ118879) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of BRAP in paraffin-embedded human lung cancer using BRAP Rabbit polyclonal antibody (STJ118879) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using BRAP Rabbit polyclonal antibody (STJ118879) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-BRAP antibody (1-200) (STJ118879)

SKU:
STJ118879

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BRAP (1-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BRAP
Gene ID: 8315
Uniprot ID: BRAP_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human BRAP (NP_006759.3).
Immunogen Sequence: MSVSLVVIRLELAEHSPVPA GFGFSAAAGEMSDEEIKKTT LASAVACLEGKSPGEKVAII HQHLGRREMTDVIIETMKSN PDELKTTVEERKSSEASPTA QRSKDHSKECINAAPDSPSK QLPDQISFFSGNPSVEIVHG IMHLYKTNKMTSLKEDVRRS AMLCILTVPAAMTSHDLMKF VAPFNEVIEQMKIIRDSTPN
Tissue Specificity Expressed in breast epithelial cell lines.
Function Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.
Protein Name Brca1-Associated Protein
Brap2
Impedes Mitogenic Signal Propagation
Imp
Ring Finger Protein 52
Ring-Type E3 Ubiquitin Transferase Brap2
Renal Carcinoma Antigen Ny-Ren-63
Database Links Reactome: R-HSA-5673000
Reactome: R-HSA-5675221
Reactome: R-HSA-6802946
Reactome: R-HSA-6802955
Reactome: R-HSA-9649948
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Brca1-Associated Protein antibody
Anti-Brap2 antibody
Anti-Impedes Mitogenic Signal Propagation antibody
Anti-Imp antibody
Anti-Ring Finger Protein 52 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Brap2 antibody
Anti-Renal Carcinoma Antigen Ny-Ren-63 antibody
Anti-BRAP antibody
Anti-RNF52 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance