Anti-BRAF antibody (STJA0033561)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2b
ImmunogenA synthetic peptide from the internal region of BRaf (V600E) , human origin.
General Information
| Short Description | Mouse monoclonal anti-BRAF for use in WB, IHC-P, ICC, IF, IP and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF/IP/ELISA |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Conjugation | Unconjugated |
| Purification | Affinity Purified |
| Dilution Range | WB-1:100-1:1000IHC-P-1:100-1:200IF-1:50-1:200ELISA-1:100-1:5000 |
| Formulation | Liquid in PBS (without Mg2+Β and Ca2+) , pH 7.4, 150 mM NaCl, 50% glycerol |
| Storage Instruction | Store at 4Β°C short term. Aliquot and store at-20Β°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | Braf |
| Gene ID | 109880 |
| Uniprot ID | BRAF_MOUSE |
| Immunogen | A synthetic peptide from the internal region of BRaf (V600E) , human origin. |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-348 by SGK1 inhibits its activity. Dephosphorylation of Ser-348 by the SHOC2-MRAS-PP1c (SMP) complex consisting of SHOC2, GTP-bound M-Ras/MRAS and the catalytic subunit of protein phosphatase 1 (PPP1CA, PPP1CB or PPP1CC).this relieves inactivation and stimulates kinase activity. Methylation by PRMT5 decreases stability and kinase activity. Ubiquitinated by RNF149.which leads to proteasomal degradation. Polyubiquitinated at Lys-615 in response to EGF. |
| Function | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. Phosphorylates PFKFB2. May play a role in the postsynaptic responses of hippocampal neurons. |
| Protein Name | Serine/Threonine-Protein Kinase B-RafProto-Oncogene B-Raf |
| Database Links | Reactome: R-MMU-1295596Reactome: -MMU-170968Reactome: -MMU-5673000Reactome: -MMU-5674135Reactome: -MMU-5674499Reactome: -MMU-5675221 |
| Cellular Localisation | NucleusCytoplasmCell MembraneColocalizes With Rgs14 And Raf1 In Both The Cytoplasm And Membranes |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase B-Raf antibodyAnti-Proto-Oncogene B-Raf antibodyAnti-Braf antibodyAnti-B-raf antibody |
Information sourced from Uniprot.org