• Immunohistochemistry analysis of paraffin-embedded rat stomach using BNIP3 Rabbit polyclonal antibody (STJ27650) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse stomach using BNIP3 Rabbit polyclonal antibody (STJ27650) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using BNIP3 antibody (STJ27650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-BNIP3 antibody (40-120) (STJ27650)

SKU:
STJ27650

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BNIP3 (40-120) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BNIP3
Gene ID: 664
Uniprot ID: BNIP3_HUMAN
Immunogen Region: 40-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-120 of human BNIP3 (NP_004043.3).
Immunogen Sequence: AFPPPAAEAHPAARRGLRSP QLPSGAMSQNGAPGMQEESL QGSWVELHFSNNGNGGSVPA SVSIYNGDMEKILLDAQHES G
Function Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway.
Protein Name Bcl2/Adenovirus E1b 19 Kda Protein-Interacting Protein 3
Cellular Localisation Mitochondrion
Mitochondrion Outer Membrane
Single-Pass Membrane Protein
Coexpression With The Eib 19-Kda Protein Results In A Shift In Nip3 Localization Pattern To The Nuclear Envelope
Colocalizes With Acaa2 In The Mitochondria
Colocalizes With Spata18 At The Mitochondrion Outer Membrane
Alternative Antibody Names Anti-Bcl2/Adenovirus E1b 19 Kda Protein-Interacting Protein 3 antibody
Anti-BNIP3 antibody
Anti-NIP3 antibody

Information sourced from Uniprot.org

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