| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BNIP1 |
| Gene ID: | 662 |
| Uniprot ID: | SEC20_HUMAN |
| Immunogen Region: | 1-200 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human BNIP1 (NP_001196.2). |
| Immunogen Sequence: | MAAPQDVHVRICNQEIVKFD LEVKALIQDIRDCSGPLSAL TELNTKVKEKFQQLRHRIQD LEQLAKEQDKESEKQLLLQE VENHKKQMLSNQASWRKANL TCKIAIDNLEKAELLQGGDL LRQRKTTKESLAQTSSTITE SLMGISRMMAQQVQQSEEAM QSLVTSSRTILDANEEFKSM SGTIQLGRKLITKYNRRELT |
| Tissue Specificity | Isoform 1 is highly expressed in heart, brain, liver skeletal muscle and pancreas. Isoform 3 is moderately expressed in placenta, lung and kidney. Isoform 4 is highly expressed in testis and small intestine. |
| Post Translational Modifications | Polyubiquitinated. 'Lys-63'-linked polyubiquitination by RNF185 increases the interaction with the autophagy receptor SQSTM1. Undergoes 'Lys-29'- and 'Lys-63'-linked polyubiquitination by RNF186 that may regulate BNIP1 localization to the mitochondrion. |
| Function | As part of a SNARE complex may be involved in endoplasmic reticulum membranes fusion and be required for the maintenance of endoplasmic reticulum organization. Also plays a role in apoptosis. It is for instance required for endoplasmic reticulum stress-induced apoptosis. As a substrate of RNF185 interacting with SQSTM1, might also be involved in mitochondrial autophagy (Probable). |
| Protein Name | Vesicle Transport Protein Sec20 Bcl2/Adenovirus E1b 19 Kda Protein-Interacting Protein 1 Transformation-Related Gene 8 Protein Trg-8 |
| Database Links | Reactome: R-HSA-6811434 |
| Cellular Localisation | Endoplasmic Reticulum Membrane Single-Pass Type Iv Membrane Protein Mitochondrion Membrane Localization To The Mitochondrion Is Regulated By Rnf186 |
| Alternative Antibody Names | Anti-Vesicle Transport Protein Sec20 antibody Anti-Bcl2/Adenovirus E1b 19 Kda Protein-Interacting Protein 1 antibody Anti-Transformation-Related Gene 8 Protein antibody Anti-Trg-8 antibody Anti-BNIP1 antibody Anti-NIP1 antibody Anti-SEC20L antibody Anti-TRG8 antibody |
Information sourced from Uniprot.org