• Western blot analysis of extracts of various cell lines, using BMP5 antibody (STJ110699) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using BMP5 antibody (STJ110699) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human prostate using BMP5 antibody (STJ110699) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using BMP5 antibody (STJ110699) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-BMP5 antibody (317-454) (STJ110699)

SKU:
STJ110699

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BMP5 (317-454) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BMP5
Gene ID: 653
Uniprot ID: BMP5_HUMAN
Immunogen Region: 317-454
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 317-454 of human BMP5 (NP_066551.1).
Immunogen Sequence: AANKRKNQNRNKSSSHQDSS RMSSVGDYNTSEQKQACKKH ELYVSFRDLGWQDWIIAPEG YAAFYCDGECSFPLNAHMNA TNHAIVQTLVHLMFPDHVPK PCCAPTKLNAISVLYFDDSS NVILKKYRNMVVRSCGCH
Tissue Specificity Expressed in the lung and liver.
Function Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cartilage and bone formation or neurogenesis. Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Can also signal through non-canonical pathway such as MAPK p38 signaling cascade to promote chondrogenic differentiation. Promotes the expression of HAMP, this is repressed by its interaction with ERFE.
Protein Name Bone Morphogenetic Protein 5
Bmp-5
Cellular Localisation Secreted
Alternative Antibody Names Anti-Bone Morphogenetic Protein 5 antibody
Anti-Bmp-5 antibody
Anti-BMP5 antibody

Information sourced from Uniprot.org

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