• Immunofluorescence analysis of Hela cells using Blk monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Flow cytometric analysis of HL-60 cells using Blk monoclonal antibody (green) and negative control (purple).
  • Western blot analysis using Blk monoclonal antibody against HEK293 (1) and BLK-hIgGFc transfected HEK293 (2) cell lysate.

Anti-BLK antibody [1E6] (STJ97869)

SKU:
STJ97869

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/IF/FC/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Tyrosine-protein kinase Blk is suitable for use in Western Blot, Immunofluorescence, Flow Cytometry and ELISA research applications.
Clonality: Monoclonal
Clone ID: 1E6
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BLK
Gene ID: 640
Uniprot ID: BLK_HUMAN
Specificity: Blk Monoclonal Antibody detects endogenous levels of Blk protein.
Immunogen: Purified recombinant fragment of human Blk expressed in E. Coli.
Post Translational Modifications Phosphorylated on tyrosine residues after antibody-mediated surface engagement of the B-cell antigen receptor (BCR). Ubiquitination of activated BLK by the UBE3A ubiquitin protein ligase leads to its degradation by the ubiquitin-proteasome pathway.
Function Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor. Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr-207'. Phosphorylates also the immunoglobulin G receptors FCGR2A, FCGR2B and FCGR2C. With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation. Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation. In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose. Phosphorylates CGAS, promoting retention of CGAS in the cytosol.
Protein Name Tyrosine-Protein Kinase Blk
B Lymphocyte Kinase
P55-Blk
Database Links Reactome: R-HSA-8939245
Reactome: R-HSA-983695
Cellular Localisation Cell Membrane
Lipid-Anchor
Present And Active In Lipid Rafts
Membrane Location Is Required For The Phosphorylation Of Cd79a And Cd79b
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Blk antibody
Anti-B Lymphocyte Kinase antibody
Anti-P55-Blk antibody
Anti-BLK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance