• Immunofluorescence analysis of HCT 116 cells using BGN Rabbit polyclonal antibody (STJ28337) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and 293T cells transfected with BGN using BGN Rabbit polyclonal antibody (STJ28337) at 1:800 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 90s.

Anti-BGN antibody (38-368) (STJ28337)

SKU:
STJ28337

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BGN (38-368) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BGN
Gene ID: 633
Uniprot ID: PGS1_HUMAN
Immunogen Region: 38-368
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 38-368 of human BGN (NP_001702.1).
Immunogen Sequence: DEEASGADTSGVLDPDSVTP TYSAMCPFGCHCHLRVVQCS DLGLKSVPKEISPDTTLLDL QNNDISELRKDDFKGLQHLY ALVLVNNKISKIHEKAFSPL RKLQKLYISKNHLVEIPPNL PSSLVELRIHDNRIRKVPKG VFSGLRNMNCIEMGGNPLEN SGFEPGAFDGLKLNYLRISE AKLTGIPKDLPETLNELHLD HNKIQAIELEDLLRYSKLYR LGLGHNQIRMIENGSLSFL
Tissue Specificity Detected in placenta (at protein level). Found in several connective tissues, especially in articular cartilages.
Post Translational Modifications The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate.
Function May be involved in collagen fiber assembly.
Protein Name Biglycan
Bone/Cartilage Proteoglycan I
Pg-S1
Database Links Reactome: R-HSA-1971475
Reactome: R-HSA-2022870
Reactome: R-HSA-2022923
Reactome: R-HSA-2024101
Reactome: R-HSA-3000178
Reactome: R-HSA-3560783
Reactome: R-HSA-3560801
Reactome: R-HSA-3595172
Reactome: R-HSA-3595174
Reactome: R-HSA-3595177
Reactome: R-HSA-4420332
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Biglycan antibody
Anti-Bone/Cartilage Proteoglycan I antibody
Anti-Pg-S1 antibody
Anti-BGN antibody
Anti-SLRR1A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance