Anti-BFAR antibody (1-140) (STJ110606)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal BFAR (1-140) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BFAR |
| Gene ID | 51283 |
| Uniprot ID | BFAR_HUMAN |
| Immunogen Region | 1-140 |
| Immunogen Sequence | MEEPQKSYVNTMDLERDEPL KSTGPQISVSEFSCHCCYDI LVNPTTLNCGHSFCRHCLAL WWASSKKTECPECREKWEGF PKVSILLRDAIEKLFPDAIR LRFEDIQQNNDIVQSLAAFQ KYGNDQIPLAPNTGRANQQM |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human BFAR (NP_057645.1). |
Additional Info
| Tissue Specificity | Expressed highly in brain, moderately in small intestine, weakly in testes and only faintly in liver and skeletal muscle. Not expressed in heart, kidney, lung and spleen. |
| Post Translational Modifications | Mediates RING-dependent self-ubiquitination leading to proteasomal degradation. |
| Function | Membrane-bound E3 ubiquitin ligase that plays a role in several processes including apoptosis regulation or reticulum endoplasmic stress. Has anti-apoptotic activity, both for apoptosis triggered via death-receptors and via mitochondrial factors. Contributes to the dynamic control of IRE1/ERN1 signaling during ER stress by inducing BAX inhibitor 1/TMBIM6 proteasomal degradation. Promotes the activation of TGF-beta signaling by mediating the 'Lys-63'-linked ubiquitination of TGFBR1 which is critical to activate the pathway. Together with NGFR, negatively regulates NF-kappa-B and JNK-related signaling pathways. Promotes the proteasome-mediated degradation of PNPLA3, a protein involveld in lipid metabolism. |
| Protein Name | Bifunctional Apoptosis RegulatorRing Finger Protein 47 |
| Database Links | |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Bifunctional Apoptosis Regulator antibodyAnti-Ring Finger Protein 47 antibodyAnti-BFAR antibodyAnti-BAR antibodyAnti-RNF47 antibody |
Information sourced from Uniprot.org