| Host: | Rabbit |
| Applications: | ELISA/WB/ICC/IHC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal anti-Beta III-Tubulin (C-Term) for use in ELISA, WB, ICC and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: | Antigen Affinity Purified |
| Dilution Range: | WB 1:2000IHC 1:500ICC 1:200 |
| Storage Instruction: | Storage at-20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C. |
| Gene Symbol: | TUBB3 |
| Gene ID: | 10381 |
| Uniprot ID: | TBB3_HUMAN |
| Immunogen Region: | C-Term |
| Specificity: | The antibody detects a 50 kDa* protein corresponding to the molecular mass of Beta III-Tubulin on SDS-PAGE immunoblots of purified brain tubulin and mouse brain tissue. |
| Immunogen: | Beta III-Tubulin synthetic peptide (coupled to KLH) corresponding to amino acid residues at the C-terminus of human Beta III-Tubulin. This sequence is not found in bI or Beta II-Tubulin isotypes, but is well conserved in Beta III-Tubulins from rat an |
| Immunogen Sequence: | Human |
| Tissue Specificity | Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. |
| Post Translational Modifications | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Glutamylation is also involved in cilia motility. Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility. Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. |
| Function | Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, alpha-beta tubulin heterodimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Plays a role in dorsal root ganglion axon projection towards the spinal cord. |
| Protein Name | Tubulin Beta-3 ChainTubulin Beta-4 ChainTubulin Beta-Iii |
| Database Links | Reactome: R-HSA-1445148Reactome: R-HSA-190840Reactome: R-HSA-190861Reactome: R-HSA-2132295Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-3371497Reactome: R-HSA-380320Reactome: R-HSA-389957Reactome: R-HSA-389960Reactome: R-HSA-389977Reactome: R-HSA-437239Reactome: R-HSA-5610787Reactome: R-HSA-5617833Reactome: R-HSA-5620924Reactome: R-HSA-5626467Reactome: R-HSA-5663220Reactome: R-HSA-6807878Reactome: R-HSA-6811434Reactome: R-HSA-6811436Reactome: R-HSA-68877Reactome: R-HSA-8852276Reactome: R-HSA-8955332Reactome: R-HSA-9609690Reactome: R-HSA-9609736Reactome: R-HSA-9619483Reactome: R-HSA-9646399Reactome: R-HSA-9648025Reactome: R-HSA-9668328Reactome: R-HSA-983189Reactome: R-HSA-9833482 |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionGrowth ConeLamellipodiumFilopodium |
| Alternative Antibody Names | Anti-Tubulin Beta-3 Chain antibodyAnti-Tubulin Beta-4 Chain antibodyAnti-Tubulin Beta-Iii antibodyAnti-TUBB3 antibodyAnti-TUBB4 antibody |
Information sourced from Uniprot.org
