• Immunofluorescence analysis of C6 cells using BCL3 Rabbit polyclonal antibody (STJ113187) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using BCL3 Rabbit polyclonal antibody (STJ113187) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using BCL3 Rabbit polyclonal antibody (STJ113187) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using BCL3 Rabbit polyclonal antibody (STJ113187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BCL3 antibody (300-400) (STJ113187)

SKU:
STJ113187

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BCL3 (300-400) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCL3
Gene ID: 602
Uniprot ID: BCL3_HUMAN
Immunogen Region: 300-400
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human BCL3 (NP_005169.2).
Immunogen Sequence: ANVNAQMYSGSSALHSASGR GLLPLVRTLVRSGADSSLKN CHNDTPLMVARSRRVIDILR GKATRPASTSQPDPSPDRSA NTSPESSSRLSSNGLLSASP S
Post Translational Modifications Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation. Activated by phosphorylation.
Function Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation.
Protein Name B-Cell Lymphoma 3 Protein
Bcl-3
Proto-Oncogene Bcl3
Cellular Localisation Nucleus
Cytoplasm
Perinuclear Region
Ubiquitination Via 'Lys-63'-Linked Ubiquitin Chains Is Required For Nuclear Accumulation
Alternative Antibody Names Anti-B-Cell Lymphoma 3 Protein antibody
Anti-Bcl-3 antibody
Anti-Proto-Oncogene Bcl3 antibody
Anti-BCL3 antibody
Anti-BCL4 antibody
Anti-D19S37 antibody

Information sourced from Uniprot.org

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