• Western blot analysis of lysates from 293 cells, treated with UV 30', using BCL-XL Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using BCL-XL Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of 293 cells using Bcl-x Polyclonal Antibody diluted at 1：500
  • Immunofluorescence analysis of HeLa cells, using BCL-XL Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using Bcl-x Polyclonal Antibody diluted at 1：500

Anti-BCL2L1 antibody (13-62 aa) (STJ91842)

SKU:
STJ91842

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Bcl-2-like protein 1 (13-62 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCL2L1
Gene ID: 598
Uniprot ID: B2CL1_HUMAN
Immunogen Region: 13-62 aa
Specificity: Bcl-x Polyclonal Antibody detects endogenous levels of Bcl-x protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human BCL-XL at the amino acid range 13-62
Post Translational Modifications Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity. Phosphorylated on Ser-62 by CDK1. This phosphorylation is partial in normal mitotic cells, but complete in G2-arrested cells upon DNA-damage, thus promoting subsequent apoptosis probably by triggering caspases-mediated proteolysis. Phosphorylated by PLK3, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Phosphorylation at Ser-49 appears during the S phase and G2, disappears rapidly in early mitosis during prometaphase, metaphase and early anaphase, and re-appears during telophase and cytokinesis. Ubiquitinated by RNF183 during prolonged ER stress, leading to degradation by the proteosome.
Function Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. Isoform Bcl-X(S) promotes apoptosis.
Protein Name Bcl-2-Like Protein 1
Bcl2-L-1
Apoptosis Regulator Bcl-X
Database Links Reactome: R-HSA-111453
Reactome: R-HSA-6785807
Reactome: R-HSA-844455
Reactome: R-HSA-9648002
Reactome: R-HSA-9692913
Reactome: R-HSA-9702518
Cellular Localisation Isoform Bcl-X(L): Mitochondrion Inner Membrane
Mitochondrion Outer Membrane
Mitochondrion Matrix
Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Cytoplasm
Cytosol
Cytoskeleton
Microtubule Organizing Center
Centrosome
Nucleus Membrane
Single-Pass Membrane Protein
Cytoplasmic Side
After Neuronal Stimulation
Translocates From Cytosol To Synaptic Vesicle And Mitochondrion Membrane In A Calmodulin-Dependent Manner
Localizes To The Centrosome When Phosphorylated At Ser-49
Alternative Antibody Names Anti-Bcl-2-Like Protein 1 antibody
Anti-Bcl2-L-1 antibody
Anti-Apoptosis Regulator Bcl-X antibody
Anti-BCL2L1 antibody
Anti-BCL2L antibody
Anti-BCLX antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance