| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BCDIN3D |
| Gene ID: | 144233 |
| Uniprot ID: | BN3D2_HUMAN |
| Immunogen Region: | 1-292 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human BCDIN3D (NP_859059.1). |
| Immunogen Sequence: | MAVPTELDGGSVKETAAEEE SRVLAPGAAPFGNFPHYSRF HPPEQRLRLLPPELLRQLFP ESPENGPILGLDVGCNSGDL SVALYKHFLSLPDGETCSDA SREFRLLCCDIDPVLVKRAE KECPFPDALTFITLDFMNQR TRKVLLSSFLSQFGRSVFDI GFCMSITMWIHLNHGDHGLW EFLAHLSSLCHYLLVEPQPW KCYRAAARRLRKLGLHDFDH FHSLAIRGDMPNQIVQILT |
| Function | O-methyltransferase that specifically monomethylates 5'-monophosphate of cytoplasmic histidyl tRNA (tRNA(His)), acting as a capping enzyme by protecting tRNA(His) from cleavage by DICER1. Also able, with less efficiently, to methylate the 5' monophosphate of a subset of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Was also reported to mediate dimethylation of pre-miR-145.however dimethylation cannot be reproduced by another group which observes a monomethylation of pre-miR-145. |
| Protein Name | Rna 5'-Monophosphate MethyltransferaseBcdin3 Domain-Containing Protein |
| Database Links | Reactome: R-HSA-203927 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Rna 5'-Monophosphate Methyltransferase antibodyAnti-Bcdin3 Domain-Containing Protein antibodyAnti-BCDIN3D antibody |
Information sourced from Uniprot.org