| Host: |
Rabbit |
| Applications: |
WB/IHC-P/IF/ICC/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
BAP1 |
| Gene ID: |
8314 |
| Uniprot ID: |
BAP1_HUMAN |
| Immunogen Region: |
1-270 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human BAP1 (NP_004647.1). |
| Immunogen Sequence: |
MNKGWLELESDPGLFTLLVE DFGVKGVQVEEIYDLQSKCQ GPVYGFIFLFKWIEERRSRR KVSTLVDDTSVIDDDIVNNM FFAHQLIPNSCATHALLSVL LNCSSVDLGPTLSRMKDFTK GFSPESKGYAIGNAPELAKA HNSHARPEPRHLPEKQNGLS AVRTMEAFHFVSYVPITGRL FELDGLKVYPIDHGPWGEDE EWTDKARRVIMERIGLATAG EPYHDIRFNLMAVVPDRRI |
| Tissue Specificity | Highly expressed in testis, placenta and ovary. Expressed in breast. levels in the placenta increase over the course of pregnancy. |
| Post Translational Modifications | Ubiquitinated: monoubiquitinated at multiple sites within its nuclear localization signal (NLS) BY UBE2O, leading to cytoplasmic retention. Able to mediate autodeubiquitination via intramolecular interactions to counteract cytoplasmic retention. Monoubiquitinated on at least 4 sites near or within its PY-NLS. |
| Function | Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the polycomb repressive deubiquitinase (PR-DUB) complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-120' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. The PR-DUB complex is an epigenetic regulator of gene expression and acts as a transcriptional coactivator, affecting genes involved in development, cell communication, signaling, cell proliferation and cell viability. Antagonizes PRC1 mediated H2AK119ub1 monoubiquitination. As part of the PR-DUB complex, associates with chromatin enriched in histone marks H3K4me1, H3K4me3, and H3K27Ac, but not in H3K27me3. Recruited to specific gene-regulatory regions by YY1. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to counteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration. Negatively regulates epithelial-mesenchymal transition (EMT) of trophoblast stem cells during placental development by regulating genes involved in epithelial cell integrity, cell adhesion and cytoskeletal organization. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase Bap1Brca1-Associated Protein 1Cerebral Protein 6 |
| Database Links | Reactome: R-HSA-5689603Reactome: R-HSA-5693565 |
| Cellular Localisation | CytoplasmNucleusChromosomeMainly NuclearBinds To ChromatinLocalizes To The Cytoplasm When Monoubiquitinated By The E2/E3 Hybrid Ubiquitin-Protein Ligase Ube2oRecruitment To Chromatin Is Dependent On Asxl1/2/3 And Recruitment To Specific Genes On Foxk1/2Nuclear Localization Is Redundantly Mediated By The Importin And Transportin SystemsTnpo1/Transportin-1 Is The Major Mediator Of Nuclear Localization |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase Bap1 antibodyAnti-Brca1-Associated Protein 1 antibodyAnti-Cerebral Protein 6 antibodyAnti-BAP1 antibodyAnti-KIAA0272 antibodyAnti-hucep-6 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
