| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S9MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:2000 IHC-P:1:200-1:800 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BAD |
| Gene ID: | 572 |
| Uniprot ID: | BAD_HUMAN |
| Immunogen Region: | 1-100 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bad (Q92934). |
| Immunogen Sequence: | MFQIPEFEPSEQEDSSSAER GLGPSPAGDGPSGSGKHHRQ APGLLWDASHQQEQPTSSSH HGGAGAVEIRSRHSSYPAGT EDDEGMGEEPSPFRGRSRSA |
| Tissue Specificity | Expressed in a wide variety of tissues. |
| Post Translational Modifications | Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Phosphorylation at Ser-99 by PKB/AKT1 is almost completely blocked by the apoptotic C-terminus cleavage product of PKN2 generated by caspases-3 activity during apoptosis. Methylation at Arg-94 and Arg-96 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-99. |
| Function | Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. |
| Protein Name | Bcl2-Associated Agonist Of Cell Death Bad Bcl-2-Binding Component 6 Bcl-2-Like Protein 8 Bcl2-L-8 Bcl-Xl/Bcl-2-Associated Death Promoter Bcl2 Antagonist Of Cell Death |
| Database Links | Reactome: R-HSA-111447 Reactome: R-HSA-111453 Reactome: R-HSA-193648 Reactome: R-HSA-198323 Reactome: R-HSA-5674400 |
| Cellular Localisation | Mitochondrion Outer Membrane Cytoplasm Colocalizes With Hif3a In The Cytoplasm Upon Phosphorylation Locates To The Cytoplasm |
| Alternative Antibody Names | Anti-Bcl2-Associated Agonist Of Cell Death antibody Anti-Bad antibody Anti-Bcl-2-Binding Component 6 antibody Anti-Bcl-2-Like Protein 8 antibody Anti-Bcl2-L-8 antibody Anti-Bcl-Xl/Bcl-2-Associated Death Promoter antibody Anti-Bcl2 Antagonist Of Cell Death antibody Anti-BAD antibody Anti-BBC6 antibody Anti-BCL2L8 antibody |
Information sourced from Uniprot.org