| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-BAAT (309-418) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:100-1:500IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
BAAT |
| Gene ID: |
570 |
| Uniprot ID: |
BAAT_HUMAN |
| Immunogen Region: |
309-418 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 309-418 of human BAAT (NP_001692.1). |
| Immunogen Sequence: |
YLFPIEEAQGQFLFIVGEGD KTINSKAHAEQAIGQLKRHG KNNWTLLSYPGAGHLIEPPY SPLCCASTTHDLRLHWGGEV IPHAAAQEHAWKEIQRFLRK HLIPDVTSQL |
| Tissue Specificity | Expressed in the gallbladder mucosa and pancreas. Expressed in hepatocytes (at protein level). |
| Function | Catalyzes the amidation of bile acids (BAs) with the amino acids taurine and glycine. More than 95% of the BAs are N-acyl amidates with glycine and taurine. Amidation of BAs in the liver with glycine or taurine prior to their excretion into bile is an important biochemical event in bile acid metabolism. This conjugation (or amidation) plays several important biological roles in that it promotes the secretion of BAs and cholesterol into bile and increases the detergent properties of BAs in the intestine, which facilitates lipid and vitamin absorption. May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs. |
| Protein Name | Bile Acid-Coa -Amino Acid N-AcyltransferaseBacatBatBile Acid-Coa ThioesteraseCholoyl-Coa HydrolaseGlycine N-CholoyltransferaseLong-Chain Fatty-Acyl-Coa Hydrolase |
| Database Links | Reactome: R-HSA-159418Reactome: R-HSA-193368Reactome: R-HSA-9033241 |
| Cellular Localisation | CytoplasmCytosolPeroxisome |
| Alternative Antibody Names | Anti-Bile Acid-Coa -Amino Acid N-Acyltransferase antibodyAnti-Bacat antibodyAnti-Bat antibodyAnti-Bile Acid-Coa Thioesterase antibodyAnti-Choloyl-Coa Hydrolase antibodyAnti-Glycine N-Choloyltransferase antibodyAnti-Long-Chain Fatty-Acyl-Coa Hydrolase antibodyAnti-BAAT antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and TOMM34 knockout (KO) HeLa cells, using [KO Validated] TOMM34 Rabbit polyclonal antibody (STJ11100836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89771/358243/STJ11100836_1__34745.1713122577.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TOMM34 knockout (KO) HeLa cells, using [KO Validated] TOMM34 Rabbit polyclonal antibody (STJ11100836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
