• Western blot analysis of HepG2, using AUH rabbit polyclonal antibody (STJ29560) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of various lysates, using AUH rabbit polyclonal antibody (STJ29560) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-AUH antibody (68-339) (STJ29560)

SKU:
STJ29560

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AUH (68-339) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AUH
Gene ID: 549
Uniprot ID: AUHM_HUMAN
Immunogen Region: 68-339
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 68-339 of human AUH (NP_001689.1).
Immunogen Sequence: SSEMKTEDELRVRHLEEENR GIVVLGINRAYGKNSLSKNL IKMLSKAVDALKSDKKVRTI IIRSEVPGIFCAGADLKERA KMSSSEVGPFVSKIRAVIND IANLPVPTIAAIDGLALGGG LELALACDIRVAASSAKMGL VETKLAIIPGGGGTQRLPRA IGMSLAKELIFSARVLDGKE AKAVGLISHVLEQNQEGDAA YRKALDLAREFLPQGPVAMR VAKLAINQGMEVDLVTGLA
Function Catalyzes the fifth step in the leucine degradation pathway, the reversible hydration of 3-methylglutaconyl-CoA (3-MG-CoA) to 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). Can catalyze the reverse reaction but at a much lower rate in vitro. HMG-CoA is then quickly degraded by another enzyme (such as HMG-CoA lyase) to give acetyl-CoA and acetoacetate. Uses other substrates such as (2E)-glutaconyl-CoA efficiently in vitro, and to a lesser extent 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA), crotonyl-CoA ((2E)-butenoyl-CoA) and 3-hydroxybutanoyl-CoA (the missing carboxylate reduces affinity to the active site). Originally it was identified as an RNA-binding protein as it binds to AU-rich elements (AREs) in vitro. AREs direct rapid RNA degradation and mRNA deadenylation. Might have itaconyl-CoA hydratase activity, converting itaconyl-CoA into citramalyl-CoA in the C5-dicarboxylate catabolism pathway. The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, an antimicrobial metabolite and immunomodulator produced by macrophages during certain infections, that can act as a vitamin B12-poisoning metabolite.
Protein Name Methylglutaconyl-Coa Hydratase - Mitochondrial
3-Mg-Coa Hydratase
Au-Specific Rna-Binding Enoyl-Coa Hydratase
Au-Binding Protein/Enoyl-Coa Hydratase
Itaconyl-Coa Hydratase
Database Links Reactome: R-HSA-70895
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Methylglutaconyl-Coa Hydratase - Mitochondrial antibody
Anti-3-Mg-Coa Hydratase antibody
Anti-Au-Specific Rna-Binding Enoyl-Coa Hydratase antibody
Anti-Au-Binding Protein/Enoyl-Coa Hydratase antibody
Anti-Itaconyl-Coa Hydratase antibody
Anti-AUH antibody

Information sourced from Uniprot.org

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