Anti-AUH antibody (68-339) (STJ29560)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal AUH (68-339) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | AUH |
| Gene ID | 549 |
| Uniprot ID | AUHM_HUMAN |
| Immunogen Region | 68-339 |
| Immunogen Sequence | SSEMKTEDELRVRHLEEENR GIVVLGINRAYGKNSLSKNL IKMLSKAVDALKSDKKVRTI IIRSEVPGIFCAGADLKERA KMSSSEVGPFVSKIRAVIND IANLPVPTIAAIDGLALGGG LELALACDIRVAASSAKMGL VETKLAIIPGGGGTQRLPRA IGMSLAKELIFSARVLDGKE AKAVGLISHVLEQNQEGDAA YRKALDLAREFLPQGPVAMR VAKLAINQGMEVDLVTGLA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 68-339 of human AUH (NP_001689.1). |
Additional Info
| Function | Catalyzes the fifth step in the leucine degradation pathway, the reversible hydration of 3-methylglutaconyl-CoA (3-MG-CoA) to 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). Can catalyze the reverse reaction but at a much lower rate in vitro. HMG-CoA is then quickly degraded by another enzyme (such as HMG-CoA lyase) to give acetyl-CoA and acetoacetate. Uses other substrates such as (2E)-glutaconyl-CoA efficiently in vitro, and to a lesser extent 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA), crotonyl-CoA ((2E)-butenoyl-CoA) and 3-hydroxybutanoyl-CoA (the missing carboxylate reduces affinity to the active site). Originally it was identified as an RNA-binding protein as it binds to AU-rich elements (AREs) in vitro. AREs direct rapid RNA degradation and mRNA deadenylation. Might have itaconyl-CoA hydratase activity, converting itaconyl-CoA into citramalyl-CoA in the C5-dicarboxylate catabolism pathway. The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, an antimicrobial metabolite and immunomodulator produced by macrophages during certain infections, that can act as a vitamin B12-poisoning metabolite. |
| Protein Name | Methylglutaconyl-Coa Hydratase - Mitochondrial3-Mg-Coa HydrataseAu-Specific Rna-Binding Enoyl-Coa HydrataseAu-Binding Protein/Enoyl-Coa HydrataseItaconyl-Coa Hydratase |
| Database Links | Reactome: R-HSA-70895Reactome: R-HSA-9914274 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Methylglutaconyl-Coa Hydratase - Mitochondrial antibodyAnti-3-Mg-Coa Hydratase antibodyAnti-Au-Specific Rna-Binding Enoyl-Coa Hydratase antibodyAnti-Au-Binding Protein/Enoyl-Coa Hydratase antibodyAnti-Itaconyl-Coa Hydratase antibodyAnti-AUH antibody |
Information sourced from Uniprot.org