| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-AUH (68-339) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
AUH |
| Gene ID: |
549 |
| Uniprot ID: |
AUHM_HUMAN |
| Immunogen Region: |
68-339 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 68-339 of human AUH (NP_001689.1). |
| Immunogen Sequence: |
SSEMKTEDELRVRHLEEENR GIVVLGINRAYGKNSLSKNL IKMLSKAVDALKSDKKVRTI IIRSEVPGIFCAGADLKERA KMSSSEVGPFVSKIRAVIND IANLPVPTIAAIDGLALGGG LELALACDIRVAASSAKMGL VETKLAIIPGGGGTQRLPRA IGMSLAKELIFSARVLDGKE AKAVGLISHVLEQNQEGDAA YRKALDLAREFLPQGPVAMR VAKLAINQGMEVDLVTGLA |
| Function | Catalyzes the fifth step in the leucine degradation pathway, the reversible hydration of 3-methylglutaconyl-CoA (3-MG-CoA) to 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). Can catalyze the reverse reaction but at a much lower rate in vitro. HMG-CoA is then quickly degraded by another enzyme (such as HMG-CoA lyase) to give acetyl-CoA and acetoacetate. Uses other substrates such as (2E)-glutaconyl-CoA efficiently in vitro, and to a lesser extent 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA), crotonyl-CoA ((2E)-butenoyl-CoA) and 3-hydroxybutanoyl-CoA (the missing carboxylate reduces affinity to the active site). Originally it was identified as an RNA-binding protein as it binds to AU-rich elements (AREs) in vitro. AREs direct rapid RNA degradation and mRNA deadenylation. Might have itaconyl-CoA hydratase activity, converting itaconyl-CoA into citramalyl-CoA in the C5-dicarboxylate catabolism pathway. The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, an antimicrobial metabolite and immunomodulator produced by macrophages during certain infections, that can act as a vitamin B12-poisoning metabolite. |
| Protein Name | Methylglutaconyl-Coa Hydratase - Mitochondrial3-Mg-Coa HydrataseAu-Specific Rna-Binding Enoyl-Coa HydrataseAu-Binding Protein/Enoyl-Coa HydrataseItaconyl-Coa Hydratase |
| Database Links | Reactome: R-HSA-70895 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Methylglutaconyl-Coa Hydratase - Mitochondrial antibodyAnti-3-Mg-Coa Hydratase antibodyAnti-Au-Specific Rna-Binding Enoyl-Coa Hydratase antibodyAnti-Au-Binding Protein/Enoyl-Coa Hydratase antibodyAnti-Itaconyl-Coa Hydratase antibodyAnti-AUH antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
