• Western blot analysis of extracts of various cell lines, using ATP5O antibody (STJ110402) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of U2OS cells using ATP5O antibody (STJ110402) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat stomach using ATP5O rabbit polyclonal antibody (STJ110402) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using ATP5O Rabbit polyclonal antibody (STJ110402) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human thyroid cancer using ATP5O rabbit polyclonal antibody (STJ110402) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat stomach using ATP5O Rabbit polyclonal antibody (STJ110402) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U2OS cells using ATP5O antibody (STJ110402) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ATP5O antibody (STJ110402) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ATP5O antibody (24-213) (STJ110402)

SKU:
STJ110402

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP5O (24-213) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP5PO
Gene ID: 539
Uniprot ID: ATPO_HUMAN
Immunogen Region: 24-213
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-213 of human ATP5O (NP_001688.1).
Immunogen Sequence: FAKLVRPPVQVYGIEGRYAT ALYSAASKQNKLEQVEKELL RVAQILKEPKVAASVLNPYV KRSIKVKSLNDITAKERFSP LTTNLINLLAENGRLSNTQG VVSAFSTMMSVHRGEVPCTV TSASPLEEATLSELKTVLKS FLSQGQVLKLEAKTDPSILG GMIVRIGEKYVDMSVKTKIQ KLGRAMREIV
Post Translational Modifications Acetylation at Lys-162 decreases ATP production. Deacetylated by SIRT3.
Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
Protein Name Atp Synthase Subunit O - Mitochondrial
Atp Synthase Peripheral Stalk Subunit Oscp
Oligomycin Sensitivity Conferral Protein
Oscp
Database Links Reactome: R-HSA-163210
Reactome: R-HSA-8949613
Cellular Localisation Mitochondrion
Mitochondrion Inner Membrane
Alternative Antibody Names Anti-Atp Synthase Subunit O - Mitochondrial antibody
Anti-Atp Synthase Peripheral Stalk Subunit Oscp antibody
Anti-Oligomycin Sensitivity Conferral Protein antibody
Anti-Oscp antibody
Anti-ATP5PO antibody
Anti-ATP5O antibody
Anti-ATPO antibody

Information sourced from Uniprot.org

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