• Western blot analysis of extracts of various cell lines, using ATPB antibody (STJ11101294) at1:20000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded human kidney using ATPB rabbit monoclonal antibody (STJ11101294) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver using ATPB rabbit monoclonal antibody (STJ11101294) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using ATPB rabbit monoclonal antibody (STJ11101294) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat colon using ATPB rabbit monoclonal antibody (STJ11101294) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Confocal imaging of HepG2 cells using ATPB rabbit monoclonal antibody (STJ11101294, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.

Anti-ATP5B antibody (230-529) [S4MR] (STJ11101294)

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STJ11101294

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ATPB (230-529) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:3000-1:20000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP5F1B
Gene ID: 506
Uniprot ID: ATPB_HUMAN
Immunogen Region: 230-529
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 230-529 of human ATPB (NP_001677.2).
Immunogen Sequence: YSVFAGVGERTREGNDLYHE MIESGVINLKDATSKVALVY GQMNEPPGARARVALTGLTV AEYFRDQEGQDVLLFIDNIF RFTQAGSEVSALLGRIPSAV GYQPTLATDMGTMQERITTT KKGSITSVQAIYVPADDLTD PAPATTFAHLDATTVLSRAI AELGIYPAVDPLDSTSRIMD PNIVGSEHYDVARGVQKILQ DYKSLQDIIAILGMDELSEE DKLTVSRARKIQRFLSQPF
Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Protein Name Atp Synthase Subunit Beta - Mitochondrial
Atp Synthase F1 Subunit Beta
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-163210
Reactome: R-HSA-2151201
Reactome: R-HSA-8949613
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Atp Synthase Subunit Beta - Mitochondrial antibody
Anti-Atp Synthase F1 Subunit Beta antibody
Anti-ATP5F1B antibody
Anti-ATP5B antibody
Anti-ATPMB antibody
Anti-ATPSB antibody

Information sourced from Uniprot.org

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