| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ATP4B |
| Gene ID: | 496 |
| Uniprot ID: | ATP4B_HUMAN |
| Immunogen Region: | 62-291 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 62-291 of human ATP4B (NP_000696.1). |
| Immunogen Sequence: | LMQTVDPYTPDYQDQLRSPG VTLRPDVYGEKGLEIVYNVS DNRTWADLTQTLHAFLAGYS PAAQEDSINCTSEQYFFQES FRAPNHTKFSCKFTADMLQN CSGLADPNFGFEEGKPCFII KMNRIVKFLPSNGSAPRVDC AFLDQPRELGQPLQVKYYPP NGTFSLHYFPYYGKKAQPHY SNPLVAAKLLNIPRNAEVAI VCKVMAEHVTFNNPHDPYEG KVEFKLKIEK |
| Post Translational Modifications | N-glycosylation is necessary for assembly and functional expression of the pump at the plasma membrane. |
| Function | The beta subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Plays a structural and regulatory role in the assembly and membrane targeting of a functionally active pump. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation of the alpha subunit that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). Interacts with the phosphorylation domain of the alpha subunit and functions as a ratchet, stabilizing the lumenal-open E2 conformation and preventing the reverse reaction of the transport cycle. |
| Protein Name | Potassium-Transporting Atpase Subunit Beta Gastric H(+/K(+ Atpase Subunit Beta Proton Pump Beta Chain |
| Database Links | Reactome: R-HSA-936837 |
| Cellular Localisation | Apical Cell Membrane Single-Pass Type Ii Membrane Protein Cell Membrane Localized In The Apical Canalicular Membrane Of Parietal Cells |
| Alternative Antibody Names | Anti-Potassium-Transporting Atpase Subunit Beta antibody Anti-Gastric H(+/K(+ Atpase Subunit Beta antibody Anti-Proton Pump Beta Chain antibody Anti-ATP4B antibody |
Information sourced from Uniprot.org