Western blot analysis of various lysates using ATP1A4 Rabbit polyclonal antibody (STJ117451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1S.

Anti-ATP1A4 antibody (1-90) (STJ117451)

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STJ117451
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:200-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP1A4
Gene ID: 480
Uniprot ID: AT1A4_HUMAN
Immunogen Region: 1-90
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human ATP1A4 (NP_653300.2).
Immunogen Sequence: MGLWGKKGTVAPHDQSPRRR PKKGLIKKKMVKREKQKRNM EELKKEVVMDDHKLTLEELS TKYSVDLTKGHSHQRAKEIL TRGGPNTVTP
Tissue Specificity Specifically expressed in testis. Found in very low levels in skeletal muscle. Expressed in mature sperm (at protein level).
Function This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Plays a role in sperm motility.
Protein Name Sodium/Potassium-Transporting Atpase Subunit Alpha-4
Na(+/K(+ Atpase Alpha-4 Subunit
Sodium Pump Subunit Alpha-4
Database Links Reactome: R-HSA-5578775
Reactome: R-HSA-936837
Reactome: R-HSA-9679191
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
In Mature Sperm
Located In The Principle Piece Of The Sperm Flagellum
Alternative Antibody Names Anti-Sodium/Potassium-Transporting Atpase Subunit Alpha-4 antibody
Anti-Na(+/K(+ Atpase Alpha-4 Subunit antibody
Anti-Sodium Pump Subunit Alpha-4 antibody
Anti-ATP1A4 antibody
Anti-ATP1AL2 antibody

Information sourced from Uniprot.org

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