• Western blot analysis of extracts of various cell lines, using Na+/K+-ATPase antibody (STJ110188) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of paraffin-embedded Mouse colon tissue using Na+/K+-ATPase Rabbit polyclonal antibody (STJ110188) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry of paraffin-embedded human liver cancer using Na+/K+-ATPase rabbit polyclonal antibody (STJ110188) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Na+/K+-ATPase in paraffin-embedded mouse kidney using Na+/K+-ATPase Rabbit polyclonal antibody (STJ110188) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human lung cancer using Na+/K+-ATPase rabbit polyclonal antibody (STJ110188) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Na+/K+-ATPase in paraffin-embedded human lung cancer using Na+/K+-ATPase Rabbit polyclonal antibody (STJ110188) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse kidney using Na+/K+-ATPase rabbit polyclonal antibody (STJ110188) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Na+/K+-ATPase in paraffin-embedded human liver cancer using Na+/K+-ATPase Rabbit polyclonal antibody (STJ110188) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Na+/K+-ATPase Rabbit polyclonal antibody (STJ110188) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ATP1A1 antibody (1-1023) (STJ110188)

SKU:
STJ110188

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Na+/K+-ATPase (1-1023) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP1A1
Gene ID: 476
Uniprot ID: AT1A1_HUMAN
Immunogen Region: 1-1023
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-1023 of human Na+/K+-ATPase (NP_000692.2).
Immunogen Sequence: MGKGVGRDKYEPAAVSEQGD KKGKKGKKDRDMDELKKEVS MDDHKLSLDELHRKYGTDLS RGLTSARAAEILARDGPNAL TPPPTTPEWIKFCRQLFGGF
Post Translational Modifications Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity.
Function This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Protein Name Sodium/Potassium-Transporting Atpase Subunit Alpha-1
Na(+/K(+ Atpase Alpha-1 Subunit
Sodium Pump Subunit Alpha-1
Database Links Reactome: R-HSA-5578775
Reactome: R-HSA-936837
Reactome: R-HSA-9679191
Cellular Localisation Basolateral Cell Membrane
Multi-Pass Membrane Protein
Cell Membrane
Sarcolemma
Cell Projection
Axon
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Sodium/Potassium-Transporting Atpase Subunit Alpha-1 antibody
Anti-Na(+/K(+ Atpase Alpha-1 Subunit antibody
Anti-Sodium Pump Subunit Alpha-1 antibody
Anti-ATP1A1 antibody

Information sourced from Uniprot.org

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