| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S8MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:50000-1:200000 IHC-P:1:400-1:4000 IF/ICC:1:100-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ATP1A1 |
| Gene ID: | 476 |
| Uniprot ID: | AT1A1_HUMAN |
| Immunogen Region: | 1-100 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Na+/K+-ATPase (P05023). |
| Immunogen Sequence: | MGKGVGRDKYEPAAVSEQGD KKGKKGKKDRDMDELKKEVS MDDHKLSLDELHRKYGTDLS RGLTSARAAEILARDGPNAL TPPPTTPEWIKFCRQLFGGF |
| Post Translational Modifications | Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity. |
| Function | This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis. |
| Protein Name | Sodium/Potassium-Transporting Atpase Subunit Alpha-1 Na(+/K(+ Atpase Alpha-1 Subunit Sodium Pump Subunit Alpha-1 |
| Database Links | Reactome: R-HSA-5578775 Reactome: R-HSA-936837 Reactome: R-HSA-9679191 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Basolateral Cell Membrane Sarcolemma Cell Projection Axon Melanosome Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names | Anti-Sodium/Potassium-Transporting Atpase Subunit Alpha-1 antibody Anti-Na(+/K(+ Atpase Alpha-1 Subunit antibody Anti-Sodium Pump Subunit Alpha-1 antibody Anti-ATP1A1 antibody |
Information sourced from Uniprot.org