• Immunohistochemistry analysis of paraffin-embedded Rat testis using ATG10 Rabbit polyclonal antibody (STJ29527) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Mouse spleen using ATG10 Rabbit polyclonal antibody (STJ29527) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human colon using ATG10 Rabbit polyclonal antibody (STJ29527) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of mouse small intestine, using ATG10 antibody (STJ29527) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ATG10 antibody (204-220) (STJ29527)

SKU:
STJ29527

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATG10
Gene ID: 83734
Uniprot ID: ATG10_HUMAN
Immunogen Region: 204-220
Specificity: A synthetic peptide corresponding to a sequence within amino acids 120-220 of human ATG10 (NP_113670.1).
Immunogen Sequence: GRPLTLKDIWEGVHECYKMR LLQGPWDTITQQEHPILGQP FFVLHPCKTNEFMTPVLKNS QKINKNVNYITSWLSIVGPV VGLNLPLSYAKATSQDERNV P
Function E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Plays a role in adenovirus-mediated cell lysis.
Protein Name Ubiquitin-Like-Conjugating Enzyme Atg10
Autophagy-Related Protein 10
Apg10-Like
Database Links Reactome: R-HSA-1632852
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Ubiquitin-Like-Conjugating Enzyme Atg10 antibody
Anti-Autophagy-Related Protein 10 antibody
Anti-Apg10-Like antibody
Anti-ATG10 antibody
Anti-APG10L antibody
Anti-PP12616 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance