• Western blot analysis of extracts of various cell lines, using ATAD3A antibody (STJ110529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using ATAD3A antibody (STJ110529) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human stomach using ATAD3A antibody (STJ110529) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human stomach using ATAD3A antibody (STJ110529) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using ATAD3A antibody (STJ110529) at dilution of 1:100 (40x lens).
  • Western blot analysis of Mouse kidney, using ATAD3A antibody (STJ110529) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ATAD3A antibody (1-240) (STJ110529)

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STJ110529

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATAD3A (1-240) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATAD3A
Gene ID: 55210
Uniprot ID: ATD3A_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ATAD3A (NP_001164006.1).
Immunogen Sequence: MSWLFGINKGPKGEGAGPPP PLPPAQPGAEGGGDRGLGDR PAPKDKWSNFDPTGLERAAK AARELEHSRYAKDALNLAQM QEQTLQLEQQSKLKEYEAAV EQLKSEQIRAQAEERRKTLS EETRQHQARAQYQDKLARQR YEDQLKQQQLLNEENLRKQE ESVQKQEAMRRATVEREMEL RHKNEMLRVEAEARARAKAE RENADIIREQIRLKAAEHRQ TVLESIRTAGTLFGEGFRA
Tissue Specificity Overexpressed in lung adenocarcinomas (at protein level).
Function Essential for mitochondrial network organization, mitochondrial metabolism and cell growth at organism and cellular level. May play an important role in mitochondrial protein synthesis. May also participate in mitochondrial DNA replication. May bind to mitochondrial DNA D-loops and contribute to nucleoid stability. Required for enhanced channeling of cholesterol for hormone-dependent steroidogenesis. Involved in mitochondrial-mediated antiviral innate immunity.
Protein Name Atpase Family Aaa Domain-Containing Protein 3a
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Mitochondrion Matrix
Mitochondrion Nucleoid
In The Mitochondrial Inner Membrane
Enriched In Sites With The Potential To Form Contacts With The Outer Membrane
The N-Terminal Domain Interacts With The Inner Surface Of The Mitochondrial Outer Membrane And The C-Terminal Domain Localizes In A Specific Matrix Compartment
Where It Is Associated With Nucleoids
Alternative Antibody Names Anti-Atpase Family Aaa Domain-Containing Protein 3a antibody
Anti-ATAD3A antibody

Information sourced from Uniprot.org

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