• Western blot analysis of extracts of various cell lines, using ATAD3A antibody (STJ110529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using ATAD3A antibody (STJ110529) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human stomach using ATAD3A antibody (STJ110529) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human stomach using ATAD3A antibody (STJ110529) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using ATAD3A antibody (STJ110529) at dilution of 1:100 (40x lens).
  • Western blot analysis of Mouse kidney, using ATAD3A antibody (STJ110529) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ATAD3A antibody (1-240) (STJ110529)

SKU:
STJ110529

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:100-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATAD3A
ATAD3B
Gene ID: 55210
83858
Uniprot ID: ATD3A_HUMAN
ATD3B_HUMAN
Immunogen Region: 1-240
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ATAD3A (NP_001164006.1).
Immunogen Sequence: MSWLFGINKGPKGEGAGPPP PLPPAQPGAEGGGDRGLGDR PAPKDKWSNFDPTGLERAAK AARELEHSRYAKDALNLAQM QEQTLQLEQQSKLKEYEAAV EQLKSEQIRAQAEERRKTLS EETRQHQARAQYQDKLARQR YEDQLKQQQLLNEENLRKQE ESVQKQEAMRRATVEREMEL RHKNEMLRVEAEARARAKAE RENADIIREQIRLKAAEHRQ TVLESIRTAGTLFGEGFRA
Tissue Specificity Q9NVI7 - Overexpressed in lung adenocarcinomas (at protein level).
Q5T9A4 - Tends to be down-regulated in differentiated cells and re-expressed in pluripotent stem cells or cancer cells (at protein level).
Alternative Antibody Names Anti-Atpase Family Aaa Domain-Containing Protein 3a antibody
Anti-ATAD3A antibody
Anti-Atpase Family Aaa Domain-Containing Protein 3b antibody
Anti-Aaa-Tob3 antibody
Anti-ATAD3B antibody
Anti-KIAA1273 antibody
Anti-TOB3 antibody

Information sourced from Uniprot.org

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