| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:200-1:2000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GET3 |
| Gene ID: | 439 |
| Uniprot ID: | GET3_HUMAN |
| Immunogen Region: | 1-348 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human ASNA1 (NP_004308.2). |
| Immunogen Sequence: | MAAGVAGWGVEAEEFEDAPD VEPLEPTLSNIIEQRSLKWI FVGGKGGVGKTTCSCSLAVQ LSKGRESVLIISTDPAHNIS DAFDQKFSKVPTKVKGYDNL FAMEIDPSLGVAELPDEFFE EDNMLSMGKKMMQEAMSAFP GIDEAMSYAEVMRLVKGMNF SVVVFDTAPTGHTLRLLNFP TIVERGLGRLMQIKNQISPF ISQMCNMLGLGDMNADQLAS KLEETLPVIRSVSEQFKDP |
| Tissue Specificity | Expressed in the epithelial cells of the liver, kidney, and stomach wall, in the adrenal medulla, in the islet cells of the pancreas, in the red pulp of the spleen, and in cardiac and skeletal muscle. |
| Function | ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1/WRB and CAMLG/GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-CAMLG receptor, and returning it to the cytosol to initiate a new round of targeting. May be involved in insulin signaling. |
| Protein Name | Atpase Get3 Arsenical Pump-Driving Atpase Arsenite-Stimulated Atpase Guided Entry Of Tail-Anchored Proteins Factor 3 - Atpase Transmembrane Domain Recognition Complex 40 Kda Atpase Subunit Harsa-I Hasna-I |
| Database Links | Reactome: R-HSA-9609523 |
| Cellular Localisation | Cytoplasm Endoplasmic Reticulum Nucleus Nucleolus |
| Alternative Antibody Names | Anti-Atpase Get3 antibody Anti-Arsenical Pump-Driving Atpase antibody Anti-Arsenite-Stimulated Atpase antibody Anti-Guided Entry Of Tail-Anchored Proteins Factor 3 - Atpase antibody Anti-Transmembrane Domain Recognition Complex 40 Kda Atpase Subunit antibody Anti-Harsa-I antibody Anti-Hasna-I antibody Anti-GET3 antibody Anti-ARSA antibody Anti-ASNA1 antibody Anti-TRC40 antibody |
Information sourced from Uniprot.org