Anti-ASCC3 antibody (1-111) (STJ110267)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ASCC3 (1-111) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ASCC3 |
| Gene ID | 10973 |
| Uniprot ID | ASCC3_HUMAN |
| Immunogen Region | 1-111 |
| Immunogen Sequence | MALPRLTGALRSFSNVTKQD NYNEEVADLKIKRSKLHEQV LDLGLTWKKIIKFLNEKLEK SKMQSINEDLKDILHAAKQI EVNCPFQKRRLDGKEEDEKM SRASDRFRGLR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-111 of human ASCC3 (NP_071374.1). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Function | ATPase involved both in DNA repair and rescue of stalled ribosomes. 3'-5' DNA helicase involved in repair of alkylated DNA: promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3, enabling ALKBH3 to process alkylated N3-methylcytosine (3mC) within double-stranded regions. Also involved in activation of the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation. Drives the splitting of stalled ribosomes that are ubiquitinated in a ZNF598-dependent manner, as part of the ribosome quality control trigger (RQT) complex. Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation. |
| Protein Name | Activating Signal Cointegrator 1 Complex Subunit 3Asc-1 Complex Subunit P200Asc1p200Helicase - Atp Binding 1Trip4 Complex Subunit P200 |
| Database Links | Reactome: R-HSA-112126 |
| Cellular Localisation | NucleusNucleus SpeckleCytoplasmCytosolColocalizes With Alkbh3 And Ascc2 In Nuclear Foci When Cells Have Been Exposed To Alkylating Agents That Cause Dna Damage |
| Alternative Antibody Names | Anti-Activating Signal Cointegrator 1 Complex Subunit 3 antibodyAnti-Asc-1 Complex Subunit P200 antibodyAnti-Asc1p200 antibodyAnti-Helicase - Atp Binding 1 antibodyAnti-Trip4 Complex Subunit P200 antibodyAnti-ASCC3 antibodyAnti-HELIC1 antibodyAnti-RQT2 antibody |
Information sourced from Uniprot.org