| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ASB2 (448-587) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ASB2 |
| Gene ID: |
51676 |
| Uniprot ID: |
ASB2_HUMAN |
| Immunogen Region: |
448-587 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 448-587 of human ASB2 (NP_057234.2). |
| Immunogen Sequence: |
MDLGCDGEPCFSCLYGNGPH PPAPQPSSRFNDAPAADKEP SVVQFCEFVSAPEVSRWAGP IIDVLLDYVGNVQLCSRLKE HIDSFEDWAVIKEKAEPPRP LAHLCRLRVRKAIGKYRIKL LDTLPLPGRLIRYLKYENTQ |
| Tissue Specificity | Isoform 1: Expressed in muscle cells. Isoform 2: Expressed in hematopoietic cells. |
| Post Translational Modifications | Isoform 1: Monoubiquitinated. Isoform 2: Not monoubiquitinated. Isoform 2: Phosphorylation at Ser-371 is required for association with FLNA and subsequent FLNA degradation. |
| Function | Substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Mediates Notch-induced ubiquitination and degradation of substrates including TCF3/E2A and JAK2. Required during embryonic heart development for complete heart looping. Required for cardiomyocyte differentiation. Isoform 1: Involved in myogenic differentiation and targets filamin FLNB for proteasomal degradation but not filamin FLNA. Also targets DES for proteasomal degradation. Acts as a negative regulator of skeletal muscle mass. Isoform 2: Targets filamins FLNA and FLNB for proteasomal degradation. This leads to enhanced adhesion of hematopoietic cells to fibronectin. Required for FLNA degradation in immature cardiomyocytes which is necessary for actin cytoskeleton remodeling, leading to proper organization of myofibrils and function of mature cardiomyocytes. Required for degradation of FLNA and FLNB in immature dendritic cells (DC) which enhances immature DC migration by promoting DC podosome formation and DC-mediated degradation of the extracellular matrix. Does not promote proteasomal degradation of tyrosine-protein kinases JAK1 or JAK2 in hematopoietic cells. |
| Protein Name | Ankyrin Repeat And Socs Box Protein 2Asb-2 |
| Database Links | Reactome: R-HSA-8951664Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmCytoskeletonStress FiberIsoform 1: CytoplasmMyofibrilSarcomereZ LineLocalizes To The Z Line In Cardiomyocytes |
| Alternative Antibody Names | Anti-Ankyrin Repeat And Socs Box Protein 2 antibodyAnti-Asb-2 antibodyAnti-ASB2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
