• STJ71016 (1µg/ml) staining of Mouse Testes lysate (A) + peptide (B) and (0. 3ug/ml) Rat Testes lysate (C) + peptide (D) 35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71016 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71016 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-Arylsulfatase A antibody (Internal) (STJ71016)

SKU:
STJ71016-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF/FC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Arylsulfatase A (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-5µg/ml
IF-Strong expression of the protein seen in the Golgi apparatus of HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ARSA
Gene ID: 410
Uniprot ID: ARSA_HUMAN
Immunogen Region: Internal
Accession Number: NP_000478.3; NP_001078897.1
Immunogen Sequence: YDLSKDPGENYN
Function Hydrolyzes cerebroside sulfate.
Protein Name Arylsulfatase A
Asa
Cerebroside-Sulfatase Cleaved Into - Arylsulfatase A Component B - Arylsulfatase A Component C
Database Links Reactome: R-HSA-1660662
Reactome: R-HSA-1663150
Reactome: R-HSA-6798695
Cellular Localisation Endoplasmic Reticulum
Lysosome
Alternative Antibody Names Anti-Arylsulfatase A antibody
Anti-Asa antibody
Anti-Cerebroside-Sulfatase Cleaved Into - Arylsulfatase A Component B - Arylsulfatase A Component C antibody
Anti-ARSA antibody

Information sourced from Uniprot.org

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