• STJ70177 (0. 5µg/ml) staining of CACO-2 (A) , (0. 1ug/ml) HeLa (B) and Jurkat (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70177 (3. 8µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • STJ70177 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70177 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-ARPC2 antibody (C-Term) (STJ70177)

SKU:
STJ70177-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/IHC/WB
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-ARPC2 (C-Term) is suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-0.5µg/ml
IHC-Recommended concentration, 3-5µg/ml
IF-Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ARPC2
Gene ID: 10109
Uniprot ID: ARPC2_HUMAN
Immunogen Region: C-Term
Accession Number: NP_005722.1; NP_690601.1
Specificity: The reported variants NP_005722.1 and NP_690601.1 represent identical protein.
Immunogen Sequence: EMKTITGKTFSSR
Protein Name Actin-Related Protein 2/3 Complex Subunit 2
Arp2/3 Complex 34 Kda Subunit
P34-Arc
Database Links Reactome: R-HSA-2029482
Reactome: R-HSA-3928662
Reactome: R-HSA-5663213
Reactome: R-HSA-8856828
Reactome: R-HSA-9664422
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Synapse
Synaptosome
Nucleus
Alternative Antibody Names Anti-Actin-Related Protein 2/3 Complex Subunit 2 antibody
Anti-Arp2/3 Complex 34 Kda Subunit antibody
Anti-P34-Arc antibody
Anti-ARPC2 antibody
Anti-ARC34 antibody
Anti-PRO2446 antibody

Information sourced from Uniprot.org

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