• Western blot analysis of extracts of various cell lines, using ARL6 antibody (STJ110568) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using ARL6 antibody (STJ110568) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human prostate using ARL6 antibody (STJ110568) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human liver damage using ARL6 antibody (STJ110568) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon using ARL6 antibody (STJ110568) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human colon using ARL6 antibody (STJ110568) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver damage using ARL6 antibody (STJ110568) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human prostate using ARL6 antibody (STJ110568) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using ARL6 antibody (STJ110568) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using ARL6 antibody (STJ110568) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ARL6 antibody (1-186) (STJ110568)

SKU:
STJ110568

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARL6 (1-186) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARL6
Gene ID: 84100
Uniprot ID: ARL6_HUMAN
Immunogen Region: 1-186
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-186 of human ARL6 (NP_115522.1).
Immunogen Sequence: MGLLDRLSVLLGLKKKEVHV LCLGLDNSGKTTIINKLKPS NAQSQNILPTIGFSIEKFKS SSLSFTVFDMSGQGRYRNLW EHYYKEGQAIIFVIDSSDRL RMVVAKEELDTLLNHPDIKH RRIPILFFANKMDLRDAVTS VKVSQLLCLENIKDKPWHIC ASDAIKGEGLQEGVDWLQDQ IQTVKT
Function Involved in membrane protein trafficking at the base of the ciliary organelle. Mediates recruitment onto plasma membrane of the BBSome complex which would constitute a coat complex required for sorting of specific membrane proteins to the primary cilia. Together with BBS1, is necessary for correct trafficking of PKD1 to primary cilia. Together with the BBSome complex and LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. May regulate cilia assembly and disassembly and subsequent ciliary signaling events such as the Wnt signaling cascade. Isoform 2 may be required for proper retinal function and organization.
Protein Name Adp-Ribosylation Factor-Like Protein 6
Bardet-Biedl Syndrome 3 Protein
Database Links Reactome: R-HSA-5620922
Cellular Localisation Cell Projection
Cilium Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasm
Cytoskeleton
Cilium Axoneme
Cilium Basal Body
Appears In A Pattern Of Punctae Flanking The Microtubule Axoneme That Likely Correspond To Small Membrane-Associated Patches
Localizes To The So-Called Ciliary Gate Where Vesicles Carrying Ciliary Cargo Fuse With The Membrane
Alternative Antibody Names Anti-Adp-Ribosylation Factor-Like Protein 6 antibody
Anti-Bardet-Biedl Syndrome 3 Protein antibody
Anti-ARL6 antibody
Anti-BBS3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance