| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ARHGEF7 |
| Gene ID: | 8874 |
| Uniprot ID: | ARHG7_HUMAN |
| Immunogen Region: | 1-260 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ARHGEF7 (NP_001106984.1). |
| Immunogen Sequence: | MNSAEQTVTWLITLGVLESP KKTISDPEGFLQASLKDGVV LCRLLERLLPGTIEKTFDAN DLYQGQNFNKVLSSLVTLNK VTADIGLGSDSVCARPSSHR IKSFDSLGSQSLHTRTSKLF QGQYRSLDMTDNSNNQLVVR AKFNFQQTNEDELSFSKGDV IHVTRVEEGGWWEGTLNGRT GWFPSNYVREVKASEKPVSP KSGTLKSPPKGFDTTAINKS YYNVVLQNILETENEYSKE |
| Post Translational Modifications | Phosphorylated by PTK2/FAK1.this promotes interaction with RAC1. Phosphorylated on Ser-694 by CaMK1.enhancement of GEF activity and downstream activation of RAC1. |
| Function | Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions. May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons. |
| Protein Name | Rho Guanine Nucleotide Exchange Factor 7Beta-PixCool-1Pak-Interacting Exchange Factor BetaP85 |
| Database Links | Reactome: R-HSA-182971Reactome: R-HSA-193648Reactome: R-HSA-3928664Reactome: R-HSA-416482Reactome: R-HSA-8980692Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013406Reactome: R-HSA-9013409Reactome: R-HSA-9013420Reactome: R-HSA-9013424Reactome: R-HSA-9619229 Q14155-1 |
| Cellular Localisation | Cell JunctionFocal AdhesionCell ProjectionRuffleCytoplasmCell CortexLamellipodiumDetected At Cell AdhesionsA Small Proportion Is Detected At Focal Adhesions |
| Alternative Antibody Names | Anti-Rho Guanine Nucleotide Exchange Factor 7 antibodyAnti-Beta-Pix antibodyAnti-Cool-1 antibodyAnti-Pak-Interacting Exchange Factor Beta antibodyAnti-P85 antibodyAnti-ARHGEF7 antibodyAnti-COOL1 antibodyAnti-KIAA0142 antibodyAnti-P85SPR antibodyAnti-PAK3BP antibodyAnti-PIXB antibodyAnti-Nbla10314 antibody |
Information sourced from Uniprot.org
