Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g ARHGAP1 antibody (STJ22672). Western blot was performed from the immunoprecipitate using ARHGAP1 antibody (STJ22672) at a dilution of 1:1000.

Anti-ARHGAP1 antibody (210-439) (STJ22672)

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STJ22672
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Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARHGAP1
Gene ID: 392
Uniprot ID: RHG01_HUMAN
Immunogen Region: 210-439
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 210-439 of human ARHGAP1 (NP_004299.1).
Immunogen Sequence: VLKYDDFLKSTQKSPATAPK PMPPRPPLPNQQFGVSLQHL QEKNPEQEPIPIVLRETVAY LQAHALTTEGIFRRSANTQV VREVQQKYNMGLPVDFDQYN ELHLPAVILKTFLRELPEPL LTFDLYPHVVGFLNIDESQR VPATLQVLQTLPEENYQVLR FLTAFLVQISAHSDQNKMTN TNLAVVFGPNLLWAKDAAIT LKAINPINTFTKFLLDHQGE LFPSPDPSGL
Tissue Specificity Ubiquitous.
Function GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate.
Protein Name Rho Gtpase-Activating Protein 1
Cdc42 Gtpase-Activating Protein
Gtpase-Activating Protein Rhogap
Rho-Related Small Gtpase Protein Activator
Rho-Type Gtpase-Activating Protein 1
P50-Rhogap
Database Links Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013405
Reactome: R-HSA-9013406
Reactome: R-HSA-9013408
Reactome: R-HSA-9013409
Reactome: R-HSA-9013423
Reactome: R-HSA-9035034
Reactome: R-HSA-9696270
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Rho Gtpase-Activating Protein 1 antibody
Anti-Cdc42 Gtpase-Activating Protein antibody
Anti-Gtpase-Activating Protein Rhogap antibody
Anti-Rho-Related Small Gtpase Protein Activator antibody
Anti-Rho-Type Gtpase-Activating Protein 1 antibody
Anti-P50-Rhogap antibody
Anti-ARHGAP1 antibody
Anti-CDC42GAP antibody
Anti-RHOGAP1 antibody

Information sourced from Uniprot.org

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