| Short Description : | Goat polyclonal antibody anti-Argininosuccinate synthetase 1 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry research applications. |
| Applications: | Pep-ELISA/WB/IF/FC/IHC |
| Host: | Goat |
| Reactivity: | Human/Mouse/Rat/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 0.5 mg/mL |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 45kDa band observed in lysates of cell lines A431 and NIH3T3 and approx.48kDa in Human and Rat Kidney and in Mouse Liver lysates (calculated MW of 46.5kDa according to Human NP_ |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | ASS1 |
| Gene ID: | 445 |
| Uniprot ID: | ASSY_HUMAN |
| Accession Number: | NP_000041.2; NP_446464.1 |
| Immunogen Region: | Internal |
| Immunogen Sequence: | ENPKNQAPPGLYTKTQD |
| Specificity: | The variants represent identical protein (NP_000041.2 and NP_446464.1). |
| Post Translational Modifications | Acetylated by CLOCK in a circadian manner which negatively regulates its enzyme activity. Deacetylated by histone deacetylases. |
| Function | One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues. |
| Protein Name | Argininosuccinate SynthaseCitrulline--Aspartate Ligase |
| Database Links | Reactome: R-HSA-70635 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Argininosuccinate Synthase antibodyAnti-Citrulline--Aspartate Ligase antibodyAnti-ASS1 antibodyAnti-ASS antibody |
Information sourced from Uniprot.org
