• STJ71128 (0. 3µg/ml) staining of A431 (A) and (1ug/ml) NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71128 (0. 01µg/ml) staining of Human Kidney (A) Mouse Liver (B) and (0. 03ug/ml) Rat Kidney (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71128 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71128 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-Argininosuccinate synthetase 1 antibody (Internal) (STJ71128)

SKU:
STJ71128-100

£312.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC/IHC
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Argininosuccinate synthetase 1 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:64000.
WB: Approx 45kDa band observed in lysates of cell lines A431 and NIH3T3 and approx.48kDa in Human and Rat Kidney and in Mouse Liver lysates (calculated MW of 46.5kDa according to Human NP_
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ASS1
Gene ID: 445
Uniprot ID: ASSY_HUMAN
Immunogen Region: Internal
Accession Number: NP_000041.2; NP_446464.1
Specificity: The variants represent identical protein (NP_000041.2 and NP_446464.1).
Immunogen Sequence: ENPKNQAPPGLYTKTQD
Post Translational Modifications Acetylated by CLOCK in a circadian manner which negatively regulates its enzyme activity. Deacetylated by histone deacetylases.
Function One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.
Protein Name Argininosuccinate Synthase
Citrulline--Aspartate Ligase
Database Links Reactome: R-HSA-70635
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Argininosuccinate Synthase antibody
Anti-Citrulline--Aspartate Ligase antibody
Anti-ASS1 antibody
Anti-ASS antibody

Information sourced from Uniprot.org

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