• Immunofluorescence analysis of NIH/3T3 cells using ARF4 Rabbit polyclonal antibody (STJ117836) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using ARF4 Rabbit polyclonal antibody (STJ117836) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ARF4 Rabbit polyclonal antibody (STJ117836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ARF4 antibody (1-180) (STJ117836)

SKU:
STJ117836

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARF4
Gene ID: 378
Uniprot ID: ARF4_HUMAN
Immunogen Region: 1-180
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ARF4 (NP_001651.1).
Immunogen Sequence: MGLTISSLFSRLFGKKQMRI LMVGLDAAGKTTILYKLKLG EIVTTIPTIGFNVETVEYKN ICFTVWDVGGQDRIRPLWKH YFQNTQGLIFVVDSNDRERI QEVADELQKMLLVDELRDAV LLLFANKQDLPNAMAISEMT DKLGLQSLRNRTWYVQATCA TQGTGLYEGLDWLSNELSKR
Function GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking.may modulate vesicle budding and uncoating within the Golgi apparatus. Part of the ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, which direct preciliary vesicle trafficking to mother centriole and ciliogenesis initiation.
Protein Name Adp-Ribosylation Factor 4
Database Links Reactome: R-HSA-5620916
Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Cellular Localisation Golgi Apparatus
Membrane
Lipid-Anchor
Alternative Antibody Names Anti-Adp-Ribosylation Factor 4 antibody
Anti-ARF4 antibody
Anti-ARF2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance