• Western blot analysis of extracts of various cell lines, using AQP9 antibody (STJ111279) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of human liver using AQP9 Rabbit polyclonal antibody (STJ111279) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human liver cancer using AQP9 rabbit polyclonal antibody (STJ111279) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of AQP9 in paraffin-embedded human liver cancer tissue using AQP9 Rabbit polyclonal antibody (STJ111279) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-AQP9 antibody (211-295) (STJ111279)

SKU:
STJ111279

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AQP9
Gene ID: 366
Uniprot ID: AQP9_HUMAN
Immunogen Region: 211-295
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 211-295 of human AQP9 (NP_066190.2).
Immunogen Sequence: SGCAMNPARDLSPRLFTALA GWGFEVFRAGNNFWWIPVVG PLVGAVIGGLIYVLVIEIHH PEPDSVFKTEQSEDKPEKYE LSVIM
Tissue Specificity Highly expressed in peripheral leukocytes. Also expressed in liver, lung, and spleen.
Function Aquaglyceroporins form homotetrameric transmembrane channels, with each monomer independently mediating glycerol and water transport across the plasma membrane along their osmotic gradient. AQP9 is the primary route for glycerol uptake in hepatocytes, supporting hepatic gluconeogenesis. It exhibits broad specificity and may transport various small, non-charged solutes, including carbamides, polyols, purines, and pyrimidines. AQP9 may also facilitate hepatic urea extrusion. Due to its permeability to lactate, AQP9 might participate in the astrocyte-to-neuron lactate shuttle, supplying neurons with energy. Additionally, AQP9 is permeable to arsenite, contributing to arsenic excretion by the liver and providing partial protection against arsenic toxicity. It is also permeable to H2O2 in vivo. Could also be permeable to ammonium.
Protein Name Aquaporin-9
Aqp-9
Aquaglyceroporin-9
Small Solute Channel 1
Database Links Reactome: R-HSA-432030
Reactome: R-HSA-432047
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Functions At The Hepatocyte Basolateral Membrane
Alternative Antibody Names Anti-Aquaporin-9 antibody
Anti-Aqp-9 antibody
Anti-Aquaglyceroporin-9 antibody
Anti-Small Solute Channel 1 antibody
Anti-AQP9 antibody
Anti-SSC1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance