• Western blot analysis of extracts of various cell lines, using AQP9 antibody (STJ111279) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of human liver using AQP9 Rabbit polyclonal antibody (STJ111279) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human liver cancer using AQP9 rabbit polyclonal antibody (STJ111279) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of AQP9 in paraffin-embedded human liver cancer tissue using AQP9 Rabbit polyclonal antibody (STJ111279) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-AQP9 antibody (211-295) (STJ111279)

SKU:
STJ111279

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AQP9 (211-295) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AQP9
Gene ID: 366
Uniprot ID: AQP9_HUMAN
Immunogen Region: 211-295
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 211-295 of human AQP9 (NP_066190.2).
Immunogen Sequence: SGCAMNPARDLSPRLFTALA GWGFEVFRAGNNFWWIPVVG PLVGAVIGGLIYVLVIEIHH PEPDSVFKTEQSEDKPEKYE LSVIM
Tissue Specificity Highly expressed in peripheral leukocytes. Also expressed in liver, lung, and spleen.
Function Forms a water channel with a broad specificity. Also permeable glycerol and urea. Mediates passage of a wide variety of small, non-charged solutes including carbamides, polyols, purines, and pyrimidines.
Protein Name Aquaporin-9
Aqp-9
Aquaglyceroporin-9
Small Solute Channel 1
Database Links Reactome: R-HSA-432030
Reactome: R-HSA-432047
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Aquaporin-9 antibody
Anti-Aqp-9 antibody
Anti-Aquaglyceroporin-9 antibody
Anti-Small Solute Channel 1 antibody
Anti-AQP9 antibody
Anti-SSC1 antibody

Information sourced from Uniprot.org

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