• Western blot analysis of various lysates, using Aquaporin-4 (AQP4) rabbit monoclonal antibody (STJ11101292) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of Aquaporin-4 (AQP4) in paraffin-embedded human lung cancer tissue using Aquaporin-4 (AQP4) rabbit monoclonal antibody (STJ11101292) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Aquaporin-4 (AQP4) in paraffin-embedded mouse brain tissue using Aquaporin-4 (AQP4) rabbit monoclonal antibody (STJ11101292) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Aquaporin-4 (AQP4) in paraffin-embedded mouse kidney tissue using Aquaporin-4 (AQP4) rabbit monoclonal antibody (STJ11101292) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Aquaporin-4 (AQP4) in paraffin-embedded mouse liver tissue using Aquaporin-4 (AQP4) rabbit monoclonal antibody (STJ11101292) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Aquaporin-4 (AQP4) in paraffin-embedded mouse lung tissue using Aquaporin-4 (AQP4) rabbit monoclonal antibody (STJ11101292) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Aquaporin-4 (AQP4) in paraffin-embedded rat brain tissue using Aquaporin-4 (AQP4) rabbit monoclonal antibody (STJ11101292) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Aquaporin-4 (AQP4) in paraffin-embedded rat lung tissue using Aquaporin-4 (AQP4) rabbit monoclonal antibody (STJ11101292) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Confocal imaging of human brain using Aquaporin-4 (AQP4) rabbit monoclonal antibody (STJ11101292, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol.
  • Confocal imaging of paraffin-embedded mouse brain using Aquaporin-4 (AQP4) rabbit monoclonal antibody (STJ11101292, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded rat brain using Aquaporin-4 (AQP4) rabbit monoclonal antibody (STJ11101292, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.

Anti-AQP4 antibody (256-323) [S2MR] (STJ11101292)

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STJ11101292

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-AQP4 (256-323) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AQP4
Gene ID: 361
Uniprot ID: AQP4_HUMAN
Immunogen Region: 256-323
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 256-323 of human Aquaporin-4 (AQP4) (NP_001641.1).
Immunogen Sequence: VEFKRRFKEAFSKAAQQTKG SYMEVEDNRSQVETDDLILK PGVVHVIDVDRGEEKKGKDQ SGEVLSSV
Tissue Specificity Detected in skeletal muscle. Detected in stomach, along the glandular base region of the fundic gland (at protein level). Detected in brain, lung and skeletal muscle, and at much lower levels in heart and ovary.
Post Translational Modifications Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamate increases conductance by 40%. Isoform 2: Palmitoylated on its N-terminal region. Isoform 1: Not palmitoylated.
Function Forms a water-specific channel. Plays an important role in brain water homeostasis and in glymphatic solute transport. Required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability.
Protein Name Aquaporin-4
Aqp-4
Mercurial-Insensitive Water Channel
Miwc
Wch4
Database Links Reactome: R-HSA-432040
Reactome: R-HSA-432047
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Endosome Membrane
Sarcolemma
Cell Projection
Activation Of The Vasopressin Receptor Avpr1a Triggers Aqp4 Phosphorylation At Ser-180 And Promotes Its Internalization From The Cell Membrane
Detected On Brain Astrocyte Processes And Astrocyte Endfeet Close To Capillaries
Alternative Antibody Names Anti-Aquaporin-4 antibody
Anti-Aqp-4 antibody
Anti-Mercurial-Insensitive Water Channel antibody
Anti-Miwc antibody
Anti-Wch4 antibody
Anti-AQP4 antibody

Information sourced from Uniprot.org

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