| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-AP2S1 (1-142) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
AP2S1 |
| Gene ID: |
1175 |
| Uniprot ID: |
AP2S1_HUMAN |
| Immunogen Region: |
1-142 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human AP2S1 (NP_004060.2). |
| Immunogen Sequence: |
MIRFILIQNRAGKTRLAKWY MQFDDDEKQKLIEEVHAVVT VRDAKHTNFVEFRNFKIIYR RYAGLYFCICVDVNDNNLAY LEAIHNFVEVLNEYFHNVCE LDLVFNFYKVYTVVDEMFLA GEIRETSQTKVLKQLLMLQS LE |
| Function | Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-FILMV (Y-X-X-Phi) and ED-X-X-X-L-LI endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the ED-X-X-X-L-LI motif. May also play a role in extracellular calcium homeostasis. |
| Protein Name | Ap-2 Complex Subunit SigmaAdaptor Protein Complex Ap-2 Subunit SigmaAdaptor-Related Protein Complex 2 Subunit SigmaClathrin Assembly Protein 2 Sigma Small ChainClathrin Coat Assembly Protein Ap17Clathrin Coat-Associated Protein Ap17Ha2 17 Kda SubunitPlasma Membrane Adaptor Ap-2 17 Kda ProteinSigma2-Adaptin |
| Database Links | Reactome: R-HSA-167590Reactome: R-HSA-177504Reactome: R-HSA-182218Reactome: R-HSA-2132295Reactome: R-HSA-3928665Reactome: R-HSA-416993 P53680-1Reactome: R-HSA-437239Reactome: R-HSA-5099900Reactome: R-HSA-5140745Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8866427Reactome: R-HSA-8964038Reactome: R-HSA-9679191 |
| Cellular Localisation | Cell MembraneMembraneCoated PitPeripheral Membrane ProteinCytoplasmic SideAp-2 Appears To Be Excluded From Internalizing Ccvs And To Disengage From Sites Of Endocytosis Seconds Before Internalization Of The Nascent Ccv |
| Alternative Antibody Names | Anti-Ap-2 Complex Subunit Sigma antibodyAnti-Adaptor Protein Complex Ap-2 Subunit Sigma antibodyAnti-Adaptor-Related Protein Complex 2 Subunit Sigma antibodyAnti-Clathrin Assembly Protein 2 Sigma Small Chain antibodyAnti-Clathrin Coat Assembly Protein Ap17 antibodyAnti-Clathrin Coat-Associated Protein Ap17 antibodyAnti-Ha2 17 Kda Subunit antibodyAnti-Plasma Membrane Adaptor Ap-2 17 Kda Protein antibodyAnti-Sigma2-Adaptin antibodyAnti-AP2S1 antibodyAnti-AP17 antibodyAnti-CLAPS2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
