| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-Ap-2 Complex Subunit Alpha-2 (590-939) is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S4629RM |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:2000-1:20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
AP2A2 |
| Gene ID: |
161 |
| Uniprot ID: |
AP2A2_HUMAN |
| Immunogen Region: |
590-939 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 590-939 of human Alpha2-adaptin/AP2A2 (NP_036437.1). |
| Immunogen Sequence: |
STDILATVLEEMPPFPERES SILAKLKKKKGPSTVTDLED TKRDRSVDVNGGPEPAPAST SAVSTPSPSADLLGLGAAPP APAGPPPSSGGSGLLVDVFS DSASVVAPLAPGSEDNFARF VCKNNGVLFENQLLQIGLKS EFRQNLGRMFIFYGNKTSTQ FLNFTPTLICSDDLQPNLNL QTKPVDPTVEGGAQVQQVVN IECVSDFTEAPVLNIQFRYG GTFQNVSVQLPITLNKFFQ |
| Tissue Specificity | Expressed in the brain (at protein level). |
| Function | Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-FILMV (Y-X-X-Phi) and ED-X-X-X-L-LI endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the ED-X-X-X-L-LI motif. |
| Protein Name | Ap-2 Complex Subunit Alpha-2100 Kda Coated Vesicle Protein CAdaptor Protein Complex Ap-2 Subunit Alpha-2Adaptor-Related Protein Complex 2 Subunit Alpha-2Alpha-Adaptin CAlpha2-AdaptinClathrin Assembly Protein Complex 2 Alpha-C Large ChainHuntingtin Yeast Partner JHuntingtin-Interacting Protein 9Hip-9Huntingtin-Interacting Protein JPlasma Membrane Adaptor Ha2/Ap2 Adaptin Alpha C Subunit |
| Database Links | Reactome: R-HSA-167590Reactome: R-HSA-177504Reactome: R-HSA-182218Reactome: R-HSA-2132295Reactome: R-HSA-3928665Reactome: R-HSA-416993 O94973-3Reactome: R-HSA-437239Reactome: R-HSA-5099900Reactome: R-HSA-5140745Reactome: R-HSA-6798695Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8866427Reactome: R-HSA-8964038Reactome: R-HSA-9679191 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmic SideMembraneCoated PitAp-2 Appears To Be Excluded From Internalizing Ccvs And To Disengage From Sites Of Endocytosis Seconds Before Internalization Of The Nascent Ccv |
| Alternative Antibody Names | Anti-Ap-2 Complex Subunit Alpha-2 antibodyAnti-100 Kda Coated Vesicle Protein C antibodyAnti-Adaptor Protein Complex Ap-2 Subunit Alpha-2 antibodyAnti-Adaptor-Related Protein Complex 2 Subunit Alpha-2 antibodyAnti-Alpha-Adaptin C antibodyAnti-Alpha2-Adaptin antibodyAnti-Clathrin Assembly Protein Complex 2 Alpha-C Large Chain antibodyAnti-Huntingtin Yeast Partner J antibodyAnti-Huntingtin-Interacting Protein 9 antibodyAnti-Hip-9 antibodyAnti-Huntingtin-Interacting Protein J antibodyAnti-Plasma Membrane Adaptor Ha2/Ap2 Adaptin Alpha C Subunit antibodyAnti-AP2A2 antibodyAnti-ADTAB antibodyAnti-CLAPA2 antibodyAnti-HIP9 antibodyAnti-HYPJ antibodyAnti-KIAA0899 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
