• Immunofluorescence analysis of NIH/3T3 cells using Annexin A2 Rabbit polyclonal antibody (STJ11103851) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of MCF7 cells using Annexin A2 Rabbit polyclonal antibody (STJ11103851) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Annexin A2 in paraffin-embedded rat heart using Annexin A2 Rabbit polyclonal antibody (STJ11103851) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Annexin A2 in paraffin-embedded mouse lung using Annexin A2 Rabbit polyclonal antibody (STJ11103851) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Annexin A2 in paraffin-embedded mouse kidney using Annexin A2 Rabbit polyclonal antibody (STJ11103851) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Annexin A2 in paraffin-embedded human liver using Annexin A2 Rabbit polyclonal antibody (STJ11103851) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Annexin A2 in paraffin-embedded human liver cancer using Annexin A2 Rabbit polyclonal antibody (STJ11103851) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Annexin A2 Rabbit polyclonal antibody (STJ11103851) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of various lysates using Annexin A2 Rabbit polyclonal antibody (STJ11103851) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ANXA2 antibody (1-339) (STJ11103851)

SKU:
STJ11103851

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Annexin A2 (1-339) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ANXA2
Gene ID: 302
Uniprot ID: ANXA2_HUMAN
Immunogen Region: 1-339
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-339 of human Annexin A2 (NP_004030.1).
Immunogen Sequence: MSTVHEILCKLSLEGDHSTP PSAYGSVKAYTNFDAERDAL NIETAIKTKGVDEVTIVNIL TNRSNAQRQDIAFAYQRRTK KELASALKSALSGHLETVIL GLLKTPAQYDASELKASMKG LGTDEDSLIEIICSRTNQEL QEINRVYKEMYKTDLEKDII SDTSGDFRKLMVALAKGRRA EDGSVIDYELIDQDARDLYD AGVKRKGTDVPKWISIMTER SVPHLQKVFDRYKSYSPYD
Post Translational Modifications Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface. ISGylated.
Function Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9. (Microbial infection) Binds M.pneumoniae CARDS toxin, probably serves as one receptor for this pathogen. When ANXA2 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen.
Protein Name Annexin A2
Annexin Ii
Annexin-2
Calpactin I Heavy Chain
Calpactin-1 Heavy Chain
Chromobindin-8
Lipocortin Ii
Placental Anticoagulant Protein Iv
Pap-Iv
Protein I
P36
Database Links Reactome: R-HSA-445355
Reactome: R-HSA-6798695
Reactome: R-HSA-75205
Reactome: R-HSA-8950505
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Basement Membrane
Melanosome
In The Lamina Beneath The Plasma Membrane
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Translocated From The Cytoplasm To The Cell Surface Through A Golgi-Independent Mechanism
Alternative Antibody Names Anti-Annexin A2 antibody
Anti-Annexin Ii antibody
Anti-Annexin-2 antibody
Anti-Calpactin I Heavy Chain antibody
Anti-Calpactin-1 Heavy Chain antibody
Anti-Chromobindin-8 antibody
Anti-Lipocortin Ii antibody
Anti-Placental Anticoagulant Protein Iv antibody
Anti-Pap-Iv antibody
Anti-Protein I antibody
Anti-P36 antibody
Anti-ANXA2 antibody
Anti-ANX2 antibody
Anti-ANX2L4 antibody
Anti-CAL1H antibody
Anti-LPC2D antibody

Information sourced from Uniprot.org

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