• Western blot analysis of extracts of various cell lines, using AMT antibody (STJ111967) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of NIH/3T3 cells using AMT Rabbit polyclonal antibody (STJ111967) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using AMT rabbit polyclonal antibody (STJ111967) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using AMT Rabbit polyclonal antibody (STJ111967) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using AMT rabbit polyclonal antibody (STJ111967) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using AMT antibody (STJ111967) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AMT antibody (107-386) (STJ111967)

SKU:
STJ111967

£112.50 - £524.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AMT
Gene ID: 275
Uniprot ID: GCST_HUMAN
Immunogen Region: 107-386
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 107-386 of human AMT (NP_001158184.1).
Immunogen Sequence: AELRPNQGTLSLFTNEAGGI LDDLIVTNTSEGHLYVVSNA GCWEKDLALMQDKVRELQNQ GRDVGLEVLDNALLALQGPT AAQVLQAGVADDLRKLPFMT SAVMEVFGVSGCRVTRCGYT GEDGVEISVPVAGAVHLATA ILKNPEVKLAGLAARDSLRL EAGLCLYGNDIDEHTTPVEG SLSWTLGKRRRAAMDFPGAK VIVPQLKGRVQRRRVGLMCE GAPMRAHSPILNMEGTKIG
Function The glycine cleavage system catalyzes the degradation of glycine.
Protein Name Aminomethyltransferase - Mitochondrial
Glycine Cleavage System T Protein
Gcvt
Database Links Reactome: R-HSA-6783984
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Aminomethyltransferase - Mitochondrial antibody
Anti-Glycine Cleavage System T Protein antibody
Anti-Gcvt antibody
Anti-AMT antibody
Anti-GCST antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance