Western blot analysis of various lysates using AMT Rabbit pAb (STJ111967) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-AMT antibody (107-386) (STJ111967)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ111967
๐Ÿšš Free UK Delivery on orders over ยฃ150
Processing The item has been added
Enquire For Bulk Order
✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

General Information

Short DescriptionRabbit polyclonal AMT (107-386) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolAMT
Gene ID275
Uniprot IDGCST_HUMAN
Immunogen Region107-386
Immunogen SequenceAELRPNQGTLSLFTNEAGGI LDDLIVTNTSEGHLYVVSNA GCWEKDLALMQDKVRELQNQ GRDVGLEVLDNALLALQGPT AAQVLQAGVADDLRKLPFMT SAVMEVFGVSGCRVTRCGYT GEDGVEISVPVAGAVHLATA ILKNPEVKLAGLAARDSLRL EAGLCLYGNDIDEHTTPVEG SLSWTLGKRRRAAMDFPGAK VIVPQLKGRVQRRRVGLMCE GAPMRAHSPILNMEGTKIG
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 107-386 of human AMT (NP_001158184.1).

Additional Info

Function The glycine cleavage system catalyzes the degradation of glycine.
Protein Name Aminomethyltransferase - Mitochondrial
Glycine Cleavage System T Protein
Gcvt
Database Links Reactome: R-HSA-6783984
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Aminomethyltransferase - Mitochondrial antibody
Anti-Glycine Cleavage System T Protein antibody
Anti-Gcvt antibody
Anti-AMT antibody
Anti-GCST antibody

Information sourced from Uniprot.org

Citations

Product Review

!