Anti-AMOTL2 antibody (201-251 aa) (STJ194903)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human AMOL2 at the amino acid range 201-251
General Information
| Short Description | Rabbit polyclonal anti-Angiomotin-like protein 2 (201-251 aa) for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | AMOTL2 |
| Gene ID | 51421 |
| Uniprot ID | AMOL2_HUMAN |
| Immunogen | Synthesized peptide derived from the human AMOL2 at the amino acid range 201-251 |
| Immunogen Region | 201-251 aa |
| Specificity | This antibody detects endogenous levels of AMOL2 at Human/Mouse/Rat |
Additional Info
| Function | Regulates the translocation of phosphorylated SRC to peripheral cell-matrix adhesion sites. Required for proper architecture of actin filaments. Plays a role in coupling actin fibers to cell junctions in endothelial cells and is therefore required for correct endothelial cell morphology via facilitating transcellular transmission of mechanical force resulting in endothelial cell elongation. Required for the anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane which facilitates organization of radial actin fiber structure and cellular response to contractile forces. This contributes to maintenance of cell area, size, shape, epithelial sheet organization and trophectoderm cell properties that facilitate blastocyst zona hatching. Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus. Participates in angiogenesis. Activates the Hippo signaling pathway in response to cell contact inhibition via interaction with and ubiquitination by Crumbs complex-bound WWP1. Ubiquitinated AMOTL2 then interacts with LATS2 which in turn phosphorylates YAP1, excluding it from the nucleus and localizing it to the cytoplasm and tight junctions, therefore ultimately repressing YAP1-driven transcription of target genes. Acts to inhibit WWTR1/TAZ transcriptional coactivator activity via sequestering WWTR1/TAZ in the cytoplasm and at tight junctions. Regulates the size and protein composition of the podosome cortex and core at myofibril neuromuscular junctions. Selectively promotes FGF-induced MAPK activation through SRC. May play a role in the polarity, proliferation and migration of endothelial cells. |
| Protein Name | Angiomotin-Like Protein 2Leman Coiled-Coil ProteinLccp |
| Database Links | Reactome: R-HSA-2028269 |
| Cellular Localisation | Recycling EndosomeCytoplasmCell ProjectionPodosomeCell Junction |
| Alternative Antibody Names | Anti-Angiomotin-Like Protein 2 antibodyAnti-Leman Coiled-Coil Protein antibodyAnti-Lccp antibodyAnti-AMOTL2 antibodyAnti-KIAA0989 antibody |
Information sourced from Uniprot.org