• Western blot analysis of lysates from Mouse lung using AMOT Rabbit polyclonal antibody (STJ11104583) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.

Anti-AMOT antibody (1000-1084) (STJ11104583)

SKU:
STJ11104583

£112.50 - £524.50
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AMOT
Gene ID: 154796
Uniprot ID: AMOT_HUMAN
Immunogen Region: 1000-1084
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1000-1084 of human AMOT (NP_001106962.1).
Immunogen Sequence: QTQAPTSAPAVAPTPAPTPT PAVAQAEVPASPATGPGPHR LSIPSLTCNPDKTDGPVFHS NTLERKTPIQILGQEPDAEM VEYLI
Tissue Specificity Expressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta.
Post Translational Modifications Polyubiquitinated by NEDD4, NEDD4L and ITCH, leading to proteasomal degradation.
Function Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions. Repressor of YAP1 and WWTR1/TAZ transcription of target genes, potentially via regulation of Hippo signaling-mediated phosphorylation of YAP1 which results in its recruitment to tight junctions.
Protein Name Angiomotin
Database Links Reactome: R-HSA-2028269 Q4VCS5-1
Reactome: R-HSA-9762292 Q4VCS5-2
Cellular Localisation Cell Junction
Tight Junction
Localized On The Cell Surface
May Act As A Transmembrane Protein
Alternative Antibody Names Anti-Angiomotin antibody
Anti-AMOT antibody
Anti-KIAA1071 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance