• Western blot analysis of extracts of HeLa cells, using Placental alkaline phosphatase (PLAP) antibody (STJ28436) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry of paraffin-embedded human placenta using Placental alkaline phosphatase (PLAP) antibody (STJ28436) at dilution of 1:100 (40x lens).
  • Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Placental alkaline phosphatase (PLAP) antibody (STJ28436). Western blot was performed from the immunoprecipitate using Placental alkaline phosphatase (PLAP) antibody (STJ28436) at a dilition of 1:1000.

Anti-ALPP antibody (260-460) (STJ28436)

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STJ28436

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ALPP (260-460) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALPP
Gene ID: 250
Uniprot ID: PPB1_HUMAN
Immunogen Region: 260-460
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 260-460 of human Placental alkaline phosphatase (PLAP) (NP_001623.3).
Immunogen Sequence: LAKRQGARYVWNRTELMQAS LDPSVTHLMGLFEPGDMKYE IHRDSTLDPSLMEMTEAALR LLSRNPRGFFLFVEGGRIDH GHHESRAYRALTETIMFDDA IERAGQLTSEEDTLSLVTAD HSHVFSFGGYPLRGSSIFGL APGKARDRKAYTVLLYGNGP GYVLKDGARPDVTESESGSP EYRQQSAVPLDEETHAGEDV A
Tissue Specificity Detected in placenta (at protein level).
Function Alkaline phosphatase that can hydrolyze various phosphate compounds.
Protein Name Alkaline Phosphatase - Placental Type
Alkaline Phosphatase Regan Isozyme
Placental Alkaline Phosphatase 1
Plap-1
Database Links Reactome: R-HSA-6811438
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Alternative Antibody Names Anti-Alkaline Phosphatase - Placental Type antibody
Anti-Alkaline Phosphatase Regan Isozyme antibody
Anti-Placental Alkaline Phosphatase 1 antibody
Anti-Plap-1 antibody
Anti-ALPP antibody
Anti-PLAP antibody

Information sourced from Uniprot.org

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