| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ALOX12 (483-663) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ALOX12 |
| Gene ID: |
239 |
| Uniprot ID: |
LOX12_HUMAN |
| Immunogen Region: |
483-663 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 483-663 of human ALOX12 (NP_000688.2). |
| Immunogen Sequence: |
YQRDDIVKGDPELQAWCREI TEVGLCQAQDRGFPVSFQSQ SQLCHFLTMCVFTCTAQHAA INQGQLDWYAWVPNAPCTMR MPPPTTKEDVTMATVMGSLP DVRQACLQMAISWHLSRRQP DMVPLGHHKEKYFSGPKPKA VLNQFRTDLEKLEKEITARN EQLDWPYEYLKPSCIENSVT I |
| Tissue Specificity | Expressed in vascular smooth muscle cells. |
| Function | Catalyzes the regio and stereo-specific incorporation of molecular oxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species. Mainly converts arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to the specific bioactive lipid (12S)-hydroperoxyeicosatetraenoate/(12S)-HPETE. Through the production of bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It can also catalyze the epoxidation of double bonds of polyunsaturated fatty acids such as (14S)-hydroperoxy-docosahexaenoate/(14S)-HPDHA resulting in the formation of (13S,14S)-epoxy-DHA. Furthermore, it may participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) like resolvin D5 ((7S,17S)-diHPDHA) and (7S,14S)-diHPDHA, that actively down-regulate the immune response and have anti-aggregation properties with platelets. An additional function involves a multistep process by which it transforms leukotriene A4/LTA4 into the bioactive lipids lipoxin A4/LXA4 and lipoxin B4/LXB4, both are vasoactive and LXA4 may regulate neutrophil function via occupancy of specific recognition sites. Can also peroxidize linoleate ((9Z,12Z)-octadecadienoate) to (13S)-hydroperoxyoctadecadienoate/ (13S-HPODE). Due to its role in regulating both the expression of the vascular endothelial growth factor (VEGF, an angiogenic factor involved in the survival and metastasis of solid tumors) and the expression of integrin beta-1 (known to affect tumor cell migration and proliferation), it can be regarded as protumorigenic. Important for cell survival, as it may play a role not only in proliferation but also in the prevention of apoptosis in vascular smooth muscle cells. |
| Protein Name | Polyunsaturated Fatty Acid Lipoxygenase Alox12Arachidonate12s-Lipoxygenase12s-Lox12s-LipoxygenaseArachidonate15s-LipoxygenaseLinoleate13s-LipoxygenaseLipoxin Synthase 12-LoPlatelet-Type Lipoxygenase 12 |
| Database Links | Reactome: R-HSA-2142696Reactome: R-HSA-2142700Reactome: R-HSA-2142712Reactome: R-HSA-9018677Reactome: R-HSA-9025106Reactome: R-HSA-9026290 |
| Cellular Localisation | CytoplasmCytosolMembraneMembrane Association Is Stimulated By Egf |
| Alternative Antibody Names | Anti-Polyunsaturated Fatty Acid Lipoxygenase Alox12 antibodyAnti-Arachidonate antibodyAnti-12s-Lipoxygenase antibodyAnti-12s-Lox antibodyAnti-12s-Lipoxygenase antibodyAnti-Arachidonate antibodyAnti-15s-Lipoxygenase antibodyAnti-Linoleate antibodyAnti-13s-Lipoxygenase antibodyAnti-Lipoxin Synthase 12-Lo antibodyAnti-Platelet-Type Lipoxygenase 12 antibodyAnti-ALOX12 antibodyAnti-12LO antibodyAnti-LOG12 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
