• Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night
  • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-ALKBH5 antibody (STJ193198)

SKU:
STJ193198

$62.10 - $305.10
Current Stock:
Host: Rabbit
Applications: IHC/IF/WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RNA demethylase ALKBH5 is suitable for use in Immunohistochemistry, Immunofluorescence and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC-P 1:50-200
WB 1:500-2000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALKBH5
Gene ID: 54890
Uniprot ID: ALKB5_HUMAN
Specificity: This antibody detects endogenous levels of human ALKBH5
Immunogen: Synthesized peptide derived from the human ALKBH5
Function Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export. Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells.
Protein Name Rna Demethylase Alkbh5
Alkylated Dna Repair Protein Alkb Homolog 5
Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 5
Database Links Reactome: R-HSA-73943
Cellular Localisation Nucleus Speckle
Alternative Antibody Names Anti-Rna Demethylase Alkbh5 antibody
Anti-Alkylated Dna Repair Protein Alkb Homolog 5 antibody
Anti-Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 5 antibody
Anti-ALKBH5 antibody
Anti-ABH5 antibody
Anti-OFOXD1 antibody

Information sourced from Uniprot.org

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