Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ALKBH4 antibody (STJ191915)

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STJ191915
£49.00 - £261.00
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Short Description :Rabbit polyclonal anti-Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4 for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents.
Applications:WB/ELISA
Host:Rabbit
Reactivity:Human/Mouse
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Polyclonal
Isotype:IgG
Conjugation:Unconjugated
Concentration:1 mg/mL
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution Range:WB 1:500-2000
ELISA 1:5000-20000
Formulation:Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:ALKBH4
Gene ID:54784
Uniprot ID:ALKB4_HUMAN
Immunogen:Synthesized peptide derived from part of the human protein
Specificity:ALKB4 Polyclonal Antibody detects endogenous levels of protein.
Function Dioxygenase that mediates demethylation of actin monomethylated at 'Lys-84' (K84me1), thereby acting as a regulator of actomyosin-processes. Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. In addition to proteins, also demethylates DNA: specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA, thereby regulating Polycomb silencing.
Protein Name Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 4
Alkylated Dna Repair Protein Alkb Homolog 4
Dna N6-Methyl Adenine Demethylase Alkbh4
Lysine-Specific Demethylase Alkbh4
Database Links
Cellular Localisation Cytoplasm
Nucleus
Nucleolus
Midbody
Associates With The Contractile Ring And Midbody
Alternative Antibody Names Anti-Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 4 antibody
Anti-Alkylated Dna Repair Protein Alkb Homolog 4 antibody
Anti-Dna N6-Methyl Adenine Demethylase Alkbh4 antibody
Anti-Lysine-Specific Demethylase Alkbh4 antibody
Anti-ALKBH4 antibody
Anti-ABH4 antibody

Information sourced from Uniprot.org

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